D2-40标记阳性淋巴管密度检测在老年非小细胞肺癌中的临床价值

The clinical value of D2-40 positive lymphatic vessel density detection in the elderly patients with non small cell lung cancer

目的观察和分析D2-40标记阳性淋巴管密度(LVD)检测在老年非小细胞肺癌(NSCLC)中的临床价值。方法选取50例NSCLC肺部肿瘤组织标本作为观察组,选取同期手术切除的良性肺病变组织标本25例作为对照组,2组标本采用免疫组化法检测D2-40表达情况,计算并比较D2-40标记阳性LVD,并分析其与临床病理特征的关系。结果肿瘤中心部位的LVD显著低于肿瘤边缘部位(P<0.05),对照组肺组织的LVD显著低于肿瘤边缘部位(P<0.05),而与肿瘤中心部位差异无统计学意义(P>0.05);肿瘤≥3 cm的标本的肿瘤边缘部位LVD显著高于肿瘤<3 cm的标本(P<0.05),随着肿瘤TNM分期的升高,肿瘤边缘部位LVD呈现升高趋势,且不同分期之间的差异有统计学意义(P<0.05),有淋巴结转移患者肿瘤边缘部位LVD显著高于无淋巴结转移的患者(P<0.05)。结论 NSCLC组织边缘部位D2-40标记阳性LVD较高,并与肿瘤大小、恶性程度和淋巴结转移有相关性,可作为预测肿瘤生长、转移和患者预后情况的提示指标。

Abstract：Objective To observe and analyze the clinical value of D240 positive lymphatic vessel density detection （LVD） in the elderly patients with non small cell lung cancer（NSCLC）. Methods 50 cases of the NSCLC tumor tissue specimens were selected as the observation group and 25 cases of benign lung lesion tissues excised in the operation dur- ing the same period were selected as the control group. The expressions of D2-40 in the specimens of the two groups were detected by immunohistochemical means. D240 positive LVDs were calculated and compared and their relationships with clinical pathological characteristics were analyzed. Results LVD at the tumor center site was significantly lower than that of the tumor edge site （ P 〈 0.05 ）. LVD of the control group was significantly lower than that of the tumor center site （ P 〈 0.05 ） ,while the difference of LVD between the control group and the tumor central site was not significant（ P 〉 0.05 ） ; LVD of the tumor specimens which was larger than 3 cm was significantly higher than that of the tumor which was less than 3 cm （ P 〈 0.05 ）. Along with the tumor TNM staging, the tumor LVD showed an increase tendency, there were signifi- cant differences between different stages（ P 〈 0.05 ）. LVD of the specimens from the patients with lymph node metastasis was significantly higher than that of the patients without lymph node metastasis （ P 〈 0.05 ）. Conclusion D2-40 positive LVD at NSCLC tumor edge site was higher, associated with the size of tumor, the malignant degree and lymph node metas- tasis and can be used to predict tumor growth, metastasis and prognosis.