Maspin mRNA在肺鳞状细胞癌组织中的表达及临床意义

Expression of Maspin mRNA in squamous cell lung carcinoma and its clinical significance

目的研究Maspin mRNA在肺鳞状细胞癌组织中的表达及其与临床病理因素之间的关系,并分析其对预后生存的影响。方法采用免疫原位杂交法检测Maspin mRNA在肺鳞状细胞癌组织及癌旁组织中的表达。结果Maspin mRNA在癌旁组织、肺鳞状细胞癌组织中的阳性表达率分别为80.00%、41.43%,差异有统计学意义(χ2=12.54,P<0.01)。Maspin mRNA的阳性表达与患者年龄、性别、淋巴结转移无关,差异无统计学意义(P>0.05),但与肿瘤直径、组织学分级、TNM分期及增殖活性Ki-67有关,差异有统计学意义(P<0.05)。Maspin mRNA阴性表达患者生存率与阳性表达患者生存率比较,差异均无统计学意义(χ2=0.03,P=0.87)。结论 Maspin mRNA表达可反映肺鳞状细胞癌的恶性程度,但不能反映患者的预后生存。

Objective To study the expression of Maspin mRNA in squamous cell lung carcinoma and its relationship with the clinicopathological factors, and to analyze its effect on the survival prognosis. Methods Maspin mRNA was detected in squamous cell lung carcinoma and paracancerous tissues by in situ hybridization. Results The positive expression rate of Maspin mRNA in paracancerous tissues and squamous cell lung carcinoma was 80.00% and 41.43% , respectively, which was statistical significance between them （X2 = 12.54, P 〈 0.01 ）. The positive expression of Maspin mRNA was not related to age, gender, lymph node metastasis, which was not statistical significance among them （ P 〉 0.05 ）, but relat- ed to tumor size, histological classification, TNM staging and Ki-67, which was statistical significance among them P 〈 0.05 ）. The survival rate of patients with positive Maspin mRNA was more than those with negative Maspin mRNA, which was not statistical significance between them （ X2 = 0.03,P = 0.87 ）. Conclusion The expression of Maspin mRNA might reflect the malignant degree of squamous cell lung carcinoma, but could not reflect patients＇ survival prognosis.