hOT7T175基因在鼻咽癌组织中的表达与意义

Expression and significance of hOT7T175 in nasopharyngeal carcinoma

目的 通过检测hOT7T175基因在鼻咽癌组织中的表达探讨其临床意义.方法 采用免疫组织化学法及反转录聚合酶链反应检测鼻咽癌和非肿瘤性鼻咽部组织hOT7T175蛋白及mRNA的表达.计数资料采用x2检验,多因素分析采用Logistic回归分析.结果 hOT7T175蛋白在鼻咽癌组阳性率为40.9％ （45/110）,低于非肿瘤性鼻咽部组织的阳性率70.5％ （31/44）,二者差异有统计学意义（x2=10.976,P=O.001）.鼻咽癌患者的淋巴结转移与hOT7T175蛋白表达关系密切,其相伴概率最小,为0.003,Wald统计量最大,为8.813,说明淋巴结转移在影响hOT7T175蛋白表达不同因素中很重要.hOT7T175 mRNA在鼻咽癌组表达率为47.1％（16/34）,低于非肿瘤性鼻咽部组织的表达率88.2％（15/17）,二者差异有统计学意义（x2=6.426,P=0.011）.影响hOT7T175 mRNA表达不同因素中鼻咽癌患者的年龄与hOT7T175 mRNA表达有关,鼻咽癌患者的淋巴结转移与hOT7T175 mRNA表达关系密切,其中淋巴结转移的相伴概率最小,为0.001,Wald统计量最大,为11.647,说明淋巴结转移在影响hOT7T175 mRNA表达不同因素中很重要.结论 hOT7T175在非肿瘤性鼻咽上皮高表达,在鼻咽癌中表达率降低.hOT7T175的表达率降低与鼻咽癌的淋巴结转移（N分期）有关,提示hOT7T175可能在鼻咽癌的转移中起重要作用.

Objective To investigate the expression and clinical significance of hOT7T175 in nasopharyngeal carcinoma （NPC）.Methods hOT7T175 expression levels were detected in nasopharyngeal carcinoma tissues and nontumorous nasopharyngeal tissues by immunohistochemistry and reverse transcription polymerase chain reaction （RT-PCR）.Categorical date were analyzed by x2 test,multi-factor analysis using logistic regression analysis.Results The positive rate of hOT7T175 protein in NPC was 40.9％ （45/110） and 70.5％ （31/44） in nontumorous nasopharyngeal tissue,and the difference was statistically significant （x2 =10.976,P =0.001）.The expression of hOT7T175 protein closely correlated with lymph node metastasis in NPC,and the smallest concomitant probability was 0.003 and the largest Wald statistic was 8.813.It was illustrated that lymph node metastasis was an important factor of affecting hOT7T175 protein expression.The positive rate of hOT7T175 mRNA in NPC was 47.1％ （16/34） and 88.2％ （15/17） in nontumorous nasopharyngeal tissue,and the difference was statistically significant （x2 =6.426,P =0.011）.The expression of hOT7T175 mRNA correlated with the age of NPC patient and lymph node metastasis.The concomitant probability of lymph node metastasis （0.001） was the smallest and the largest Wald statistic was 11.647.It was illustrated that lymph node metastasis was an important factor of affecting hOT7T175 mRNA expression.Conclusion hOT7T175 highly expresses in nontumorous nasopharyngeal tissue and lowly expresses in NPC.The low expression of hOT7T175 is correlated with lymph node metastasis of NPC,which indicates that hOT7T175 may play an important role in the metastasis of NPC.