摘要
目的测定止咳定喘丸中苦杏仁苷的含量,为止咳定喘丸的质量研究提供依据。方法采用高相液相色谱(HPLC)法测定止咳平喘丸中苦杏仁的活性成分苦杏仁苷的含量;使用Agilent SB-C18(4.6 mm×250 mm,5μm)色谱柱;以乙腈-0.1%磷酸(8∶92)为流动相,流量1.0 ml/min。结果建立了HPLC法测定止咳定喘丸中苦杏仁苷的含量,苦杏仁苷进样量在0.0824~2.0600μg范围内,线性关系良好(r=0.9995),平均回收率为99.03%,RSD=2.25%(n=6)。结论该方法准确、重复性好,可用于止咳平喘丸的质量控制。
Objective To establish a method for amygdalin in Zhikedingchuan pills,in order to provide the basis for Zhikedingehuan pills quality research. Methods The content of amygdalin was determind by HPLC was carried on with a Agilent SB-C18（4.6 mm^250 mm,5 ~m） eolumn,acctonitrile-0.1% phosphate （8：9：2） as the mobile phase at the flow rate of 1.0 ml/min. Rusults Establish a method of HPLC for amygdalin in Zhikedingchuan pills.Amygdalin showed good linear relationship in the range of 0.0824-2.0600 I, Lg （r=0.9995）,the average recovery was 99.03%,RSD=2.25% （n=6）. Conclusion The method is accurate with a good reproducibility,and suitable for quality control of Zhikedingchuan pills.
出处
《中国当代医药》
2014年第4期7-9,共3页
China Modern Medicine
