桑叶总黄酮不同纯化工艺的比较

Comparative studies on different methods of purification of total flavones from Mulberry leaf

目的比较桑叶总黄酮不同的纯化工艺,为找到一种总黄酮含量>15%,简便可行,廉价且适于生产的纯化工艺。方法采用D101型、AB-8型和JD-1型大孔吸附树脂吸附法,乙酸乙酯萃取法,醇提水沉法,醇提-水沉-醇沉法6种方法对桑叶总黄酮进行纯化,以总黄酮的收率和含量为考察指标进行评价。结果 6种方法制备的总黄酮的百分含量依次为:50.3%、48.5%、55.7%、82.1%、10.8%、18.5%,产品收率依次为:2.37%、2.42%、2.28%、0.45%、18.21%、15.93%。结论采用醇提-水沉-醇沉法即可得到符合要求的桑叶总黄酮。

Objective To compare the purification effects of different purification techniques of total flavones from Mulberry leaf.In order to find a purification technology which is cheap,simple,feasible and suitable for the production of total flavones whose purity is more than 15%.Methods Six purifying methods,including adsorption with macroreticular adsorption resin D101,AB-8,JD-l,extraction with ethylacetate,alCohol sedimentation method,alcohol sedimentation and alcohol precipitation method,were adopted,and the amound and purity of total flavones collected were compared.Results By using the above-mentioned six methods,the percentages of the contents of total flavones was 50.3%,48.5%,55.7%,82.1%, 10.8% and 18.5% respectively,the recoveries of products was 2.37%,2.42%,2.28%,0.45%,18.21% and 15.93% respective- ly.Conclusion Using the alcohol sedimentation and alcohol precipitation method can get total flavones up to requirement.