白癜风患者淋巴细胞对自身抗原表位肽的免疫应答研究

The cellular immunity against autoantigens in vitiligo patients

目的探讨进展期白癜风患者的细胞免疫功能。方法对门诊收集的102例进展期白癜风患者进行HLA-A位点分型,应用ELISPOT实验,采用已知的5条白癜风相关的表位肽段刺激患者外周血单个核细胞,通过斑点形成细胞数目分析患者外周血中针对特异抗原的细胞免疫应答水平,分析比较治疗前后的变化,确定白癜风患者细胞免疫应答与疾病进展有无相关性。结果 38.24%(39/102)白癜风患者为HLA-A*0201限制性。HLA-A*0201限制性患者外周血淋巴细胞针对自身表位肽段有高水平的免疫应答。经过有效治疗后,免疫应答有所下降。结论汉族白癜风患者外周血淋巴细胞对已知的白癜风自身抗原表位存在特异性细胞免疫应答,这种免疫应答水平可能与疾病活动相关。

Objective To investigate the cellular autoimmunity in 102 Chinese patients with progressive vitiligo. Methods HLA-A alleles were tested among 102 progressive vitiligo patients. Five antigenic peptides originated from tyrosinase, gp100 and Melan A/MART-1 were applied in the ELISPOT assays to detect the cellular autoimmunity of 102 patients. We compared the ELISPOT results of the progressive vitiligo patients before and after therapy to elucidate the correlation of cellular autoimmunity and disease activity. Results 38.24%（39/102） of the vitiligo patients were HLA-A＊0201-restricted who showed signiifcantly higher immune reactivity towards antigenic peptides than patients with other HLA-A alleles. After 1 and 3 months therapy, patients showed lower cellular autoimmunity towards antigenic peptides. Conclusion Cellular autoimmunity towards the reported antigenic peptides exists in progressive vitiligo patients of Chinese population and correlates with disease activity.