3Koshiol J,Wang E,Zhao Y,et al. Strengths and limitations of la-boratory procedures for microRNA detection [ J]. Cancer Epidemi-ol Biomarkers Prev, 2010,19(4) :907 -11.
4Derveaux S,Vandesompele J,Hellemans J. How to do successfulgene expression analysis using real-time PCR [ J ]. Methods,2010,50(4):227 -30.
5Benes V, Castoldi M. Expression profiling of microRNA using re-al-time quantitative PCR, how to use it and what is available[ J].Methods, 2010,50(4) :244 一9.
6Leite K R, Canavez J M, Reis S T,et al. miRNA analysis offormalin-fixed paraffin embedded and fresh-frozen tissue [ J ]. UrolOncol, 2011,29(5) :533 -7.
7Stahlberg A, Kubista M, Pfafil M. Comparison of reverse tran-scriptases in gene expression analysis[ J]. Clin Chem,2004,50(9):1678 -80.
8Hurley J, Roberts D, Bond A, et al. Stem-loop RT-qPCR for mi-croRNA expression profiling[ J ]. Methods Mol Biol, 2012 ,822 :33 -52.
9Mestdagh P, Feys T, Bernard N,et al. High-through put stem-loop RT-qPCR miRNA expression profiling using minute amountsof input RNA[ J]. Nucleic Acids Res,2008,36(21) :el43.
10Tichopad A, Kitchen R, Riedmaier I,et al. Design and optimiza-tion of reverse transcription quantitative PCR experiments [ J ].Clin Chem, 2009,55(10) :1816 -23.
