东亚砂藓组织培养技术方法研究

Research on Tissue Culture Technology of Racomitrium japonicum

以东亚砂藓（Racomitrium japonicum）配子体为外植体，比较了不同消毒液对消毒效果的影响，对接种消毒外植体的培养基进行了尝试性筛选，并研究了蔗糖浓度对东亚砂藓配子体生长的影响。结果显示，将东亚砂藓配子体用2％洗洁精溶液浸泡数分钟后，再用0．02％的升汞处理45-60S的消毒效果最佳；有机培养基是无菌外植体接种的最佳培养基；3％的蔗糖更有利于无菌外植体产生的原丝体团和幼嫩配子体的生长。

Abstract： The effects of different disinfectants on explants of Racomitrium japonicum were studied here. Suitable inoculation media were screened tentatively, and the effects of sucrose concentrations on growth of R. japonicum gametophytes were investigated. The results showed that a concentration of 0.02% HgCI2 for 45-60 seconds was optimal for initiation cultures from fragments of gametophytes pre-immersed in 2% detergent for a period of time. The suitable inoculation medium for sterile explants was organic culture medium MS. Protonemal colonies and young gametophytes then grew well when 3% sucrose was added. Key words： Racomitrium japonicum; Explants; Sterilization method; Medium; Sucrose concentration