卵巢上皮性癌组织Smad4与P16表达及其与微血管密度的相关性研究

Detection of Expressions of Smad4 and P16 and Their Relationship with MVD in Ovarian Carcinoma

目的探讨卵巢上皮性癌组织中Smad4,P16的表达以及微血管密度(MVD)计数,探讨三者之间的关系。方法随机选取100例卵巢肿瘤患者组织样本,其中卵巢上皮性癌50例、交界性卵巢肿瘤30例、良性卵巢肿瘤20例。应用免疫组化以及组织芯片技术检测各组织样本Smad4,P16和CD34的表达,通过观察CD34来测定MVD。结果Smad4和P16的阳性率以及MVD在卵巢上皮性癌组织中分别为34.0%(17/50),54%(27/50),19.0±8.9,在交界性卵巢肿瘤组织中分别为43.3%(13/30),76.7%(23/30),15.0±6.3,在卵巢良性肿瘤组织中分别为100%(20/20),100%(20/20),9.9±4.7;三者在两组间的差异均有统计学意义(P<0.05);卵巢上皮性癌Smad4和P16的表达均与MVD呈负相关(P<0.05),Smad4与P16的表达呈正相关(P<0.01)。结论Smad4和P16的基因表达缺失可能与卵巢上皮性癌的发生有较大相关性,作用原理可能与血管形成有关。

Objective To detect the expressions of Smad 4and P16 and the microvasculardensity （ MVD） in Ovarian carcinoma tissues so as to understand the relationship among Smad 4,P16 and MVD.Methods One hundred cases of ovarian cancer patient samples were chosen,including 50 cases of epithelial ovarian cancerApplication of immunohistochemical and detection of 50 cases of ovarian carcino-ma tissue microarray technology group ,30 cases of ovarian tumor tissue border sex and Smad 4 in 20 cases of ovarian benign tumor tissue ,the expression of P16 and CD34,by observing the CD34 to determine MVD,30 cases of borderline ovarian tumors ,20 cases of benign ovarian tumors,and Immunohistochemistry and Tissue Microarray Technology were applied to detect the expression of Smad 4,P16 and CD34.MVD was measured by observing the expression of CD 34.Results Smad4 and P16 positive rate and MVD in ovarian carcinoma tissues was 34.0%（17/50）,54%（27/50）,19.0 ±8.9,border in ovarian tumor tissues was 43.3%（13/30）,76.7%（23/30） and 15.0 ±6.3,and in ovarian benign tumor tissues was 100%（20/20）,100%（20/20） and 9.9 ±4.7;The three differences between the two groups hadve statistical sig-nificance（P〈0.05）;Smad4 ovarian carcinoma and the expression of P 16 in ovarian carcinoma were and negatively correlated with MVD （P〈0.05）,and the positive correlation was showed between the expression of P 16 and Smad4（P〈0.01）.Conclusions In Ovarian carcinoma the loss of Smad4 and P16 may play an important role in pancreatic carcinogenesis .Its underlying mechanisms might be associated with angio-genesis.