无绿藻对小鼠Th17细胞分化的影响

The effect of prototheca on the differentiation of mice Th17 cells

目的 研究无绿藻是否能够影响小鼠Th17细胞的分化,影响Th17细胞分泌相关的细胞因子.方法 体外培养无绿藻,用尼龙毛柱法分离培养小鼠脾脏T淋巴细胞,按照1∶5的比例将两者在transwell培养板上共培养,培养2h、4h、8h、12 h、24 h、48 h,分别收集T淋巴细胞,留取培养上清液.流式细胞技术检测Th17细胞表型,ELISA法检测培养上清液中IL-17的水平.结果 从共培养2h开始,随着共培养时间的延长Th17细胞所占比例逐渐降低,共培养8h后趋于稳定（P＜0.05）;从共培养2h开始,培养上清液中IL-17的水平逐渐升高,8h后出现下降,24h后趋于稳定（P＜0.05）.结论 在与T细胞共培养的条件下,无绿藻抑制了小鼠Th17细胞的分化,但是在最初2～8h可以促进Th17相关细胞因子IL-17的释放.

Objective To study if prototheca could influence the differentiation of mice Th17 cells and the secretion of Th17 associated cytokines.Methods Prototheca was cultured in vitro,and T lymphocytes were isolated from spleen of mice by nylon wool column,and cultured in vitro.Prototheca and T cells were co-cultured in transwell plate with a ratio of 1∶ 5.Cells were culture for 2 h,4 h,8 h,12 h,24 h,48 h,and then T cells and supernatant were collected respectively,and the ratio of Th17 cell was analyzed with flow cytometry,and the level of IL-17 in the supernatant was detected with ELISA.Results From 2 h on,with the prolonged incubation time,the proportion of Th17 cells decreased gradually,and it became stable 8 h later （P ＜ 0.05） ; the level of IL-17 in the supernatant increased at earlier 2-8 h,and decreased from 8 h on,and it became stable 24 h later （P ＜ 0.05）.Conclusion Being co-cultured with T cells,Prototheca could inhibit the differentiation of mice Th17 cells,but it could promote the secretion of IL17 at the earlier 2-8 h.