28种药物联合氟康唑对白念珠菌体外抑菌作用的初步研究

In vitro interactions of 28 kinds of drugs with fluconazole against Candida albicans

目的从常用免疫抑制剂、降血脂药、抗抑郁药、抗生素及非甾体类抗炎药中筛选出可能与氟康唑具有协同增效作用的药物。方法将5类28种药物配制成相同浓度(100μg/mL)后分别单独及与低浓度氟康唑联合加入白念珠菌耐药菌株(100、103)及敏感菌株(sc5314、y0109)菌悬液中,24 h(敏感菌)及48 h(耐药菌)后观察菌液澄清情况,菌液完全澄清为"+",混浊为"-",并重复上述实验3次。结果舍曲林等7种药物在该浓度下单用即可抑制所有实验菌株生长,与氟康唑合用后抑菌作用更加显著;利福喷丁等6种药物在该浓度下单用只能抑制敏感菌株生长,但与低浓度氟康唑合用后对耐药菌株也有抑制作用。结论舍曲林、利福喷丁等药物与氟康唑联合后对白念珠菌可能具有协同抑制作用。

Objective To screen the drugs which may have synergistic effects with fluconazole from 28 kinds of drugs including immunosuppressive agents,antilipemic agents,antidepressants,antibiotics and non-steroidal anti-inflammatory drugs.Methods 28 kinds of drugs within 5 main types were formulated intothe uniform concentrations of 100 μg/mL,then were dripped into the fungal suspensions of Candida albicans including fluconazole-resistant strains （100,103） and sensitive strains （sc5314,y0109）,singly or together with fluconazole of low concentrations,respectively.Then the clarity of the fungal suspensions were observed respectively,the sensitive strains after 24 h,while the resistant strains after 48 h.＂ ＋ ＂ was noted for the completely clarified cases,while ＂-＂ for the turbid cases accordingly.The test was repeated three times simultaneously.Results Sertraline and other 6 kinds of drugs could inhibit the growth of all the Candida albicans under the concentration of 100 μg/mL singly,which had more significant effects when combined with fluconazole.Rifapentine and other 5 kinds of drugs could only inhibit the growth of the sensitive strains under that concentration singly,but were still effective on the resistant strains when combined with fluconazole of low concentrations.Conclusion Some drugs such as sertraline,rifapentine may have synergistic inhibition effect on Candida albicans with fluconazole.