替米沙坦对新疆哈萨克族高血压患者外周血T淋巴细胞活化增殖后IKCal钾通道电流的阻滞作用

Effects of telmisartan on IKCal potassium channel after T-lymphocyte activation and proliferation in peripheral blood of hypertensive patients in Xinjiang Kazakh

目的研究替米沙坦对新疆哈萨克族高血压患者外周血T淋巴细胞活化增殖后IKCal钾通道电流的阻滞作用。方法随机数字表法选择2012年8—12月在新疆医科大学第一附属医院高血压门诊未经降压药物治疗的新疆哈萨克族高血压患者30例，分离其外周血T淋巴细胞进行体外培养增殖。将培养增殖后T淋巴细胞分为空白对照组、替米沙坦组及TRAM-34组，给予相应分组处理后继续培养0、24、48h。采用膜片钳技术记录T淋巴细胞活化后IKCal钾通道电生理特性的变化。结果在不同处理条件下，T淋巴细胞活化后IKCal钾通道电生理特性表现各不相同。同一时间点上各组间两两比较，显示替米沙坦组干预24h和48h的IKCal钾通道峰电流、峰值电流密度明显小于空白对照组[24h：（835±117）pA比（1471±255）pA，（213±61）pA／pF比（388±129）pA／pF；48h：（631±142）pA比（1555±383）pA，（155．4-54）pA／pF比（388±114）pA／pF，均P〈0．01]。替米沙坦对T淋巴细胞活化后IKCal钾通道干预0、24、48h的阻断率分别为6．8％、45．1％、60．1％。结论替米沙坦可以阻断新疆哈萨克族高血压患者T淋巴细胞IKCal钾通道，提示替米沙坦可能通过阻断T淋巴细胞活化时IKCal钾通道发挥抗炎作用，其意义有待进一步研究。

Objective To explore the effects of Telmisartan on IKCal potassium channel after T- lymphocyte activation and proliferation in peripheral blood of hypertensive patients in Xinjiang Kazakh. Methods Peripheral blood T cells in vitro culture were isolated from 30 Xinjiang Kazakh outpatients without antihypertensive drug therapy. They were randomly selected from our hypertension clinic from August 2012 to December 2012. The proliferated T lymphocytes were divided into control, telmisartan and TRAM-34 groups. After culturing for 0, 24, 4g h after corresponding treatments, the patch-clamp technique was employed to record the electrophysiological changes of IKCal potassium channel of T lymphocytes. Results Under different treatment conditions, the IKCal potassium channel showed different electrophysiological changes. Pairwise comparison was made among the groups on the same time. For the telmisartan group, IKCal potassium channel peak current, peak current density of intervention 24 h and 48 h were significantly reduced compared with the control group （24 h：（835± 117）vs（ 1 471 ± 255） pA, （213 ± 61 ） vs （388± 129） pA/pF;48h：（631±142） vs （1 555 ±383） pA, （155±54） vs （388 ±114） pA/pF, all P〈 0.01 ）. And the blocking rates of 0 h, 24 h and 48 h of telmisartan on IKCal potassium channel were 6.8% , 45.1% and 60.1% respectively. Conclusion Telmisartan can block the IKCal potassium channel of T lymphoeytes in peripheral blood of hypertensive patients in Xinjiang Kazakh. It suggests that telmisartan may play an anti-inflammatory effect by blocking the IKCal potassium channels of T lymphocyte activation.