基于线粒体COⅠ和Cytb基因序列研究粉蝶科七属间的系统发生关系

Phylogenetic relationships among seven Pieridae genera based on partial sequences of the COⅠ and Cytb genes

【目的】为了探讨粉蝶科Pieridae 7属的系统进化关系。【方法】基于线粒体COⅠ(609 bp)和Cytb(393 bp)基因部分序列,以眼蝶科的2个物种为外类群,运用UPGMA和ME法重建分子系统树。【结果】联合基因构建的分子系统树显示:外群牧女珍眼蝶Coenonympha amaryllis(Cramer)和阿芬眼蝶Aphantopus hyperantu(Linnaeus)构成一独立支系,可以作为外群;云粉蝶属和粉蝶属姊妹关系构成一分支,亲缘关系较近;襟粉蝶属与钩粉蝶属形成姐妹关系,亲缘关系较近。【结论】成功重建了粉蝶科7属的系统进化关系。

[Objectives] The phylogenetic relationships among 7 genera of butterflies in the Pieridae were investigated. [Methodsl Based on partial sequences of the CO Ⅰ （ 609 bp） and Cytb （ 393 bp） genes, and with 2 species of Satyrinae as the outgroup, phylogenetic relationships were analyzed and reconstructed with the UPGMA and ME methods. [Results] The molecular phylogenetic trees showed that Coenonympha amaryllis and Aphantopus hyperantu were an outgroup; Pieris, Pontia, Anthocharis, Gonepteryx, Aporis, Colias were, a group; Pontia and Pieris were a sister-group, Aporia and Mesapia were a group with a close relationship, Gonepteryx and Anthocharis were a sister-group.