前列腺素E1对肝动脉栓塞后肝纤维化进展的防治

Prostaglandin E1 for prevention and treatment of liver fibrosis progression after hepatic arterial embolization in a rabbit model

目的:探讨前列腺素E1(prostaglandin E1,PGE1)在家兔肝纤维化模型基础上行肝动脉栓塞后肝纤维化进展的防治.方法:选取四氯化碳(CCl4)肝纤维化动物模型家兔30只,模拟肝癌行肝右动脉碘化油栓塞,随机分为3组:A组经导管向肝右动脉注入生理盐水1 mL;B组经导管向肝右动脉注入碘化油1 mL;C组经导管向肝右动脉注入碘化油1mL+PGE1 0.2 mL,术后第2、4、6、8天抽取静脉血行肝纤维化血清学检测,术后第8天处死动物行肝组织病理学检测.结果:建立家兔肝纤维化模型,行肝动脉栓塞术中加入PGE1显著降低血清学透明质酸(hyaluronic acid,HA)、层粘连蛋白(laminin,LN)、Ⅲ型前胶原(procollagenⅢ,PCⅢ)及Ⅳ型胶原(collagenⅣ,Ⅳ-C)水平及减轻肝组织病理学损伤.结论:PGE1对肝纤维化肝动脉碘化油栓塞术后肝纤维化进展具有保护作用.

AIM： To investigate whether prostaglandin E1 （PGE1） has preventive and therapeutic effects on liver fibrosis progression after transcatheter hepatic arterial embolization in a rabbit model. METHODS： Thirty rabbits were used to induce liver fibrosis with carbon tetrachloride （CCl4）, and transcatheter right hepatic arterial embolization was performed. The rabbits were divided into three groups randomly （groups A, B and C） and infused with 1 mL of 0.9% saline, 1 mL of lipiodol and 1 ml of lipiodol plus 0.2 mL of PGE1 via the right hepatic artery, respectively. Serum levels of hyaluronic acid （HA）, laminin （LN）, procollagen Ⅲ （PCⅢ）, and collagen Ⅳ （Ⅳ-C） on postoperative days 2, 4, 6 and 8 were measured, and the animals were killed on postoperative day 8 to detect pathological changes in liver tissues. RESULTS： Liver fibrosis was successfully induced in rabbits, and addition of PGE1 in hepatic arterial embolization significantly reduced serum levels of HA, LN, PCⅢ and Ⅳ-C, and alleviated hepatic histological abnormalities. CONCLUSION： PGE1 can prevent liver fibrosis progression in rabbits after transcatheter hepatic arterial embolization with lipiodol.