复合病因造模法制备功能性消化不良大鼠模型

Establishment of a rat model of functional dyspepsia by a compound method

目的:采用夹尾刺激+不规则喂养复合法复制功能性消化不良(functional dyspepsia,FD)大鼠模型.方法:24只大鼠随机分为正常组、模型1组、模型2组,每组8只.模型1组进行夹尾激惹刺激,对后2组大鼠进行夹尾激惹刺激及不规则喂养干预.每周进行敞箱实验(open-field法)、糖水消耗实验的测定,4 wk后测定胃排空率及小肠推进率.结果:4 wk后,模型1组、模型2组大鼠均出现扎堆、靠边,不喜活动,攻击性减少,易受惊吓,毛发干枯不顺,粪便变软等表现.4 wk后剖腹取胃,正常组胃黏膜无明显潮红、肿胀、糜烂等病变,模型1组及模型2组胃内残留物较多,胃黏膜稍潮红.模型1组、模型2组大鼠糖水消耗、敞箱实验水平运动次数、胃排空率及小肠推进率均较正常组显著减低(P<0.05),而模型2组大鼠糖水消耗、敞箱实验水平运动次数、胃排空率及小肠推进率均显著低于模型1组(P<0.05),模型2组大鼠敞箱实验垂直运动次数低于模型1组,但无显著性差别.结论:用夹尾刺激+不规则喂养复合方法建立F D大鼠模型较单纯夹尾刺激法更能理想的体现出FD患者抑郁、胃肠动力减慢的疾病特点.

AIM： To establish the rat model of functional dyspepsia （FD） by a method combining constantly squeezing the tail with hunger or overeating. METHODS： Twenty-four rats were randomly divided into a control group and two model groups （n = 8 for each group）. In one model group, FD was induced by constantly squeezing rat tails. In the other model group, FD was induced by constantly squeezing rat tails plus hunger or overeating. Open-field test was performed weekly. After four weeks, gastric emptying and intestinal transit function were detected. RESULTS： Rats in the two model groups all showed pad batch, margination, less movement, reduced aggressiveness, easy to rag, withered hair, and loose stools. The rats were sacrificed and laparotomized four weeks later, and gastric mucosa in the control group had no significant flushing, swelling, erosions or other changes, while the model groups had much gastric contents and flushing. The sugar consumption, the scores for horizontal motion in the open-field test, and the rates of gastric emptying and intestinal transit function were all decreased in the model groups compared to the control group （all P 〈 0.05）, and the decreases were more significance in the compound model group （all P 〈 0.05）. The scores for vertical motion in the open-field test showed no significance difference between the two model groups. CONCLUSION： FD induced by constantly squeezing rat tails plus hunger or overeating can better reflect disease features of FD such as depression and decreased gastrointestinal motility than that induced by constantly squeezing the tails alone.