氟伏沙明对N2a细胞mTOR、Camk2γ及GSK-3β表达的影响

Effects of fluvoxamine on expressions of mTOR,Camk2γ and GSK-3β in N2a cells

目的探讨氟伏沙明对N2a细胞雷帕霉素靶蛋白(mTOR)、钙/钙调素依赖性蛋白激酶2γ(Camk2γ)及糖原合酶激酶3β(GSK-3β)表达的影响及其可能机制。方法将培养成功的N2a细胞随机分为3组,每组6个复孔,分别加入DMEM(D组)、氟伏沙明0.5μmol/L(F组)和BD1047(σ1受体阻断剂)0.2μmol/L+氟伏沙明0.5μmol/L(BF组)培养48h。Western blot检测各组mTOR、Camk2γ及GSK-3β的表达。结果与D组相比,F组mTOR及Camk2γ表达上调,GSK-3β表达下调(P<0.01);与F组相比,BF组mTOR及Camk2γ表达下调,GSK-3β表达上调(P<0.01)。结论氟伏沙明的药理作用可能与激活σ1受体活性有关。

Objective To study the effects and underlying mechanisms of fluvoxamine on the expressions of mammalian target of rapamycin（mTOR）,Caz＋/calmodulin-dependent protein kinase 27 （Camk27） and glycogen synthase kinase 313（GSK-3） in cultured N2a cells. Methods The cultured N2a cells were randomly divided into 3 groups with 6 samples each. The NZa cells in group D were treated with DMEM, those in group F with fluvoxamine 0. 5 vmol/L, and those in group BF with BD1047（al receptor antagonist） 0.2 vmol/L plus fluvoxamine 0.5 /μmol/L. The expressions of mTOR,Camk27 and GSK-3 in the cultured N2a cells were detected by Western blot two days after incubation, respectively. Results Compared with group D, group F showed a significant increase in the expressions of mTOR and Camk27, and a decrease in the expression of GSK-3[^（P〈0. 01）. Compared with group F,group BF showed a significant decrease in the expressions of roTOR and Camk27, and an increase in the expression of GSK-3 （P〈0. 01）. Conclusion The pharmacological property of fluvoxamine is probably related to the activation of receptor.