摘要
目的研究色素播散综合征(PDS)和色素性青光眼(PG)致病基因糖蛋白神经调节肽B相关基因(Gpnmb)在BXD重组近交系(BXD RI)小鼠眼组织的表达差异及其基因调控网络。方法采用Affymetrix基因芯片检测确定68个品系的BXD RI小鼠眼组织中Gpnmb基因的表达差异。采用基因表达数量性状基因座(eQTL)分析方法,结合GeneNetwork网站上的在线分析工具,研究差异表达的特征,进而构建Gpnmb基因调控网络。结果 Gpnmb在BXD RI眼组织中平均表达水平为10.92,不同品系小鼠表达量存在差异。Gpnmb基因为顺式作用的eQTL,End3基因是其下游候选基因。基于和Gpnmb高度相关基因的基因网络得以构建。结论构建Gpnmb基因调控网络,为PDS和PG发病机制的深入研究提供线索。
Objective To investigate the differential expression and genetic regulatory network analysis of Gpnmb gene that contributes to pathogenesis of pigmentary dispersion syndrome（PDS） and pigmentary glaucoma （PG） in the eyes of BXD recombinant inbred （BXD RI） mice. Methods Affymetrix microarray system was used to detect the differential expression of Gpnmb gene in the eyes of C57BL/6J（B6） ,DBA/2J（D2） and BXD RI mice. Expression quantitative trait loci（eQTL） and online analytical tool in GeneNetwork were approached to characterize the differential expression and construct a genetic regulatory network of Gpnmb gene Results The average expression level of Gpnmb gene in different strains of BXD RI mice was 10.92 with a range from 7.91 to 12.33. Gpmnb gene was a cis-eQTL, and End3 was its candidate downstream gene. Moreover, a genetic regulatory network of Gpnmb gene was constructed by forty genes highly correlated with Gpnmb gene. Conclusion The genetic regulatory network of Gpnmb has been constructed, which may provide a clue for further studvir the pathoenesis of PDS and PG.
出处
《江苏医药》
CAS
北大核心
2014年第2期138-140,I0001,共4页
Jiangsu Medical Journal
基金
江苏省自然科学基金(BK2008186)
南通市社会事业创新与示范计划(HS2011005)
