山西地区淋球菌质粒谱型分析

Analysis on Plasmid Profiles of Neisseria Gonorrhoeae in Shanxi Province

目的了解山西地区淋球菌流行株的质粒谱,探讨淋球菌质粒谱与耐药谱间的关系。方法采用碱裂解法提取山西地区54株淋球菌的质粒,分析其质粒谱型。结果 54株淋球菌中,51株有可靠的分析结果,其中49株检测出携带有质粒,检出率96.08%。以7.4kb+4.2kb,39.5kb+7.4kb+4.2kb及42.5kb+39.5kb+7.4kb+4.2kb三种类型居多,占82.35%。结论质粒图谱显示山西地区的质粒谱型有别于国内其他地区,具有地区特殊性,产青霉素酶淋球菌(PPNG)与7.4kb质粒密切相关。

Objective To survey plasmid profiles of Neisseria gonorrhoeaes in Shanxi, probe the relationship between the plasmid profiles and resistance of neisseria gonorrhoeaes. Methods The plasmids were extracted by al kaline lysis technique. Analysis the plasmid profiles of neisseria gonorrhoeaes in Shanxi. Resuts Fifty-one strains of fifty-four strains has the reliable resuhs. The detection rate of plasmid is 96.08%. Three main types were 7.4kb ＋ 4.2kb, 39.5kb ＋ 7.4kb ＋ 4.2kb and 42.5kb ＋ 39.5kb ＋ 7.4kb ＋ 4.2kb accounting for 82.35% of total isolates. Conclusion The plasmid profiles of neisseria gonorrhoeaes in Shanxi are different of the other areas. It has its specificity. PPNG was correlated to the 7.4 kb plasmid.