摘要
目的探讨髓源性抑制细胞(MDSCs)负性共刺激分子(B7-HI)的表达及在增龄相关免疫抑制功能中的作用。方法健康C57BL/6老年(12月龄)及青年(4~8周龄)小鼠各18只。通过流式细胞分析法测定各组小鼠MDSCs上负性协同刺激因子的表达水平,并分别通过免疫磁珠分选法及小鼠淋巴结研磨方法,选得MDSCs及T细胞。T细胞增殖干预实验分5组进行:T细胞羟基荧光素二醋酸盐琥珀酰亚胺脂(CFSE)未标记组;T细胞CFSE标记组;T细胞标记CFSE+老年MDSCs组;T细胞标记CFSE+青年MDSCs组以及T细胞标记CFSE+老年MDSCs+B7-H1阻断抗体组,观察B7-HI在MDSCs抑制T细胞增殖过程中的作用。结果与青年组比较,老年组MDSCsB7-H1的表达明显增高(t=3.27,P〈O.05)。老年组MDSCs能明显抑制T细胞的增殖(t=5.42,P〈O.05),而青年组MDSCs对T细胞的干预作用不明显(t=0.64,P〉0.05),老年MDSCs加B7-HI阻断抗体组T细胞增殖被明显逆转,差异有统计学意义(£=8.28,P〈O.05)。结论表达于MDSCs表面的B7-H1分子可能是增龄相关MDSCs发挥免疫抑制功能的重要调节因子。
Objective To explore the expression of negative costimulatory molecule B7-H1 of myeloid derived suppressor cells(MDSCs) and its roles in age-related immunosuppressive functions. Methods 36 C57BL/6 mice were divided into 2 groups: the elderly group(12-month-oId, n= 18) and the young group(4-8-week-old, n=18). The expressive level of negative costimulatory molecules of MDSCs in the two groups was measured by flow cytometry(FCM). Besides, MDSCs were sorted by using MDSC isolation kit and T cells were obtained by grinding lymph nodes. To explore the influence of B7-H1 on the MDSCs immunosuppressive functions, T cell proliferation and intervention assay was conducted using the five following groups: T cells group, T cells + CFSE group [T cells with carboxyfluorescein diaeetate succinimidyl ester (CFSE), T cells + CFSE eocultured with MDSCs from the young group, T cells + CFSE cocultured with MDSCs from the elderly group, and T cells + CFSE cocultured with MDSCs from elderly group and anti-BT-H1 blocking antibody. The effect of B7-H1 on T cell proliferation in MDSCs were observed. Results The expression level of BT-H1 of the MDSCs was significantly increased in the elderly group as compared with the young group(t= 3.27, P〈0.05). T cell proliferation assay revealed that T cells were remarkably suppressed by MDSCs in elderly group as compared to the youth group(t=5.42, P%0.05). The suppression of T cells by MDSCs was dramatically reversed by anti-B7-H1 blocking antibody in elderly group(t= 8.28, P〈0.05). Conclusions BT-H1, a molecule expressed on the surface of MDSCs, plays a key role in the age-related immunosuppressive function of MDSCs.
出处
《中华老年医学杂志》
CAS
CSCD
北大核心
2014年第2期197-200,共4页
Chinese Journal of Geriatrics
基金
江苏省自然科学基金(BK2012607)
苏州市科技发展项目(SYS201116)
关键词
抑制因子
免疫
抗原
CD
免疫耐受
Suppressor factors, immunolagic
Antigens, CD
Immune tolerance