miR-124 rs531564基因多态性与宫颈癌遗传易感有密切关系

Correlation analysis between miR-124 rs531564 polymorphisms and susceptibility to cervical cancer

目的探讨广东地区汉族妇女miR-124 rs531564基因多态性与宫颈癌遗传易感的关系,为宫颈癌的预防和治疗提供新的思路。方法利用聚合酶链式反应-连接酶检测反应(PCR-LDR)技术,对107例宫颈癌患者和208名健康对照个体的miR-124基因rs531564多态位点进行分型。采用非条件逻辑回归分析统计该多态位点与宫颈癌易感的相关性,计算相对危险度的比值比(OR)及95%置信区间(CI)。结果 HPV感染在病例组中的分布频率显著高于正常对照组(93.1%vs 16.8%,P<0.001),提示HPV感染是广东地区宫颈癌发病的重要危险因素。等位基因的结果显示,miR-124 rs531564 G等位基因在病例组中的分布频率显著低于正常对照组(8.0%vs 15.1%,P=0.014)。与CC基因型相比,携带G等位基因的个体(CG+GG)显著降低患宫颈癌的风险(OR=0.47,95%CI=0.26-0.88,P=0.017)。而且G等位基因降低个体患宫颈癌风险的趋势在年龄≥45岁的人群中更加明显(OR=0.28,95%CI=0.10-0.76,P=0.012)。结论 miR-124基因rs531564基因多态性与广东地区汉族妇女宫颈癌的遗传易感有密切关系,G等位基因可能是降低宫颈癌发病风险的重要因素。

Objective To investigate the correlation between miR-124 rs531564 polymorphisms and the susceptibility to cervical cancer in Chinese Han women in Guangdong Province. Methods The genotypes of miR-124 rs531564 polymorphism were determined using polymerase chain reaction-based ligase detection reaction （PCR-LDR） in 107 cervical cancer patients and 208 healthy female blood donors. The correlation between the polymorphism and the susceptibility to cervical cancer was evaluated using unconditional logistic regression analysis. Results The incidence of HPV infection in the patients （93.1%） was much higher than that in the control subjects （16.8%, P＆lt;0.001）, suggesting the importance of HPV infection as a critical risk factor for cervical cancer. The G allele of miR-124 rs531564 polymorphism in the cervical cancer patients was much less frequent than that in the controls （8.0%vs 15.1%, P=0.014）, suggesting its possible role as a protective allele. Compared with those carrying CC genotype, individuals carrying the CG and GG genotypes showed a significantly reduced risk for cervical cancer （OR=0.47, 95%CI=0.26-0.88, P=0.017）, and this protective role of the G allele was more prominent in older women （≥45 years old） （OR=0.28, 95% CI=0.10-0.76, P=0.012）. Conclusion miR-124 rs531564 polymorphism may play a role in cervical cancer susceptibility in Chinese Han women, and G allele is associated with a reduced risk of cervical cancer.