不同频率振动应力对破骨细胞特异性基因及骨保护素／核因子κB受体活化因子配体表达的影响

Effects of different frequency of vibration strain on the expressions of osteoclast-specific genes and osteoprotegerin/receptor of activator for nuclear factor-κB ligand in osteoclast

目的:观察不同频率振动应力对RAW264.7细胞体外诱导分化过程中,其特异性基因及骨保护素(OPG)/核因子κB受体活化因子配体(RANKL)表达的影响。方法:应用复合振动仪,将不同频段3—10Hz、15—35Hz、35—45Hz、50—70Hz和70—90Hz振动应变分别作用于体外诱导分化的RAW264.7细胞,分别为B、C、D、E、F组,未进行振动干预组为A组,振动应变加载3天和6天时,应用RT-PCR方法检测破骨细胞特异性基因(TRAP、MMP-9和CATK)与OPG/RANKL的表达水平。结果:不同振动频率组破骨细胞特异性基因表达水平逐渐减低,同时B、C、D组逐渐上调OPG基因表达,而RANKL基因的表达逐渐下调。结论:不同频率振动应力均抑制RAW264.7细胞向成熟破骨细胞增殖分化。

Objective： To investigate effects of different ic genes and osteoprotegerin（OPG）/receptor cells in vitro induced differentiation process. frequency of vibration strain on the of activator for nuclear factor-r,B expression of osteoclast-specif- ligand（RANKL） in RAW264.7 Method： RAW264.7 cells were subjected to vibration strain with different frequency [3--10Hz （B-goup）, 15-35Hz（C-group）, 35--45Hz（D-group）, 50---70Hz（E-group）,70---90Hz（F-group）] and similar induced fluid. A-group cells were not subjected to vibration strain.The expressions of osteoclast-specific genes（TRAP, MMP-9 and CATK） and OPG/RANKL in osteoclasts were analyzed by semi-quantative RT-PCR. Result： In different frequency vibration groups, the expression levels of osteoclast-specific genes reduced gradu- ally, at the same time in B,C,D group vibration strain promote the expression of OPG mRNA and inhibit the expression of RANKL mRNA gradually. Conclusion： Different frequency of vibration strain can inhibit proliferation and differentiation of RAW264.7 cells into osteoclasts.