白细胞介素-27在小鼠病毒性心肌炎中的表达

Expressions of IL-27 in the coxsackievirus B3-induced mice viral myocarditis

目的：研究小鼠病毒性心肌炎（VMC）模型中白细胞介素（IL）-27基因和蛋白的表达,探究其在VMC发病中的作用及意义。方法：VMC组用柯萨奇病毒B3（CVB3）感染Balb/c小鼠建立VMC模型,注射100TCID 50病毒液0.1ml;对照组注射等量磷酸盐缓冲液（PBS）。在注射后的第0、1、2、3、4和6周应用逆转录-聚合酶链反应检测两组小鼠心肌组织中IL-27亚基p28及EBI3的mRNA表达,酶联免疫双夹心抗体法检测血清中IL-27蛋白的表达。结果：与对照组比较,VMC组小鼠心肌组织中IL-27p28mRNA水平自第1周开始升高,第2周时达峰值,并至少维持至第6周（均P〈0.05）;VMC组第1~6周各时点血清IL-27蛋白均明显高于对照组（均P〈0.05）;与对照组同时点比较,VMC组各时点小鼠心肌组织中IL-27EBI3mRNA差异均无统计学意义。结论：IL-27在VMC小鼠中高表达,提示IL-27可能参与VMC的发病机制。

Objective：To investigate the expression of interleukin-27（IL-27）in the coxsackievirus B3-induced mice viral myocarditis（VMC）.Method：BALB/c mice were intraperitoneally infected with CVB 3for establishing VMCmodels.Control mice were treated with phosphate-buffered saline（PBS）.On 0,1,2,3,4,6week after injection,expressions of serum IL-27were analyzed by enzyme linked immosorbent assay（ELISA）.Productions of cardiac IL-27p28and EBI3mRNA were measured by real time-polymerase chain reaction（RT-PCR）.Result： Levels of cardiac IL-27p28mRNA obviously increased in VMC mice on week 1after infection,peaked on week 2, and highly persisted to at least week 6（all P0.05）.Compared with control group,serum IL-27protein was higher in VMC group from week 1to week 6（all P0.05）.There was no significant difference production of cardiac IL-27EBI3mRNA was found between VMC and control group throughout the course of the experiment.Conclusion：The increased levels of IL-27may play an important role in the pathogenesis of CVB3-induced mice VMC.