侵袭性垂体腺瘤超微结构及MMP-9、VEGF表达特点与瘤细胞侵袭的关系

Relationship between ultrastructure, expression of MMP-9 and VEGF and invasiveness in pituitaryadenomas

目的研究侵袭性垂体腺瘤超微形态特点及MMP-9、VEGF与肿瘤侵袭性生长的关系。方法选取30例侵袭性垂体腺瘤和30例非侵袭性垂体腺瘤临床手术活检标本，应用电子显微镜观察两者超微结构的差异，包括粗面内质网、核糖体、线粒体数目、核异形及核仁等，并结合MMP-9及VEGF的表达，综合分析垂体腺瘤的侵袭性。结果侵袭性垂体腺瘤在电镜下可见粗面内质网增多，核糖体丰富，胞核形态怪异，核仁硕大；核仁出现率、核异形率、核浆比例及线粒体数目均比非侵袭性垂体腺瘤组显著增高（P〈0．01）。免疫组化结果证实，MMP-9及VEGF在侵袭性垂体腺瘤中呈强阳性表达，在非侵袭性垂体腺瘤组织内呈阴性或弱阳性低表达。结论侵袭性垂体腺瘤电镜观察结果及免疫组化结果提示瘤细胞的超微结构与侵袭性生长有关。

Objective To study the relationship between the ultrastructural characteristics, MMP-9 and VEGF and invasiveness in pituitary adenomas. Methods To observe the difference between the two groups of pituitary adenomas （30 cases in invasive group, 30 cases in non-invasive group ） in the uhrastructural characteristics by electron microscope, including the rough endoplasmic reticulum, the number of mitochondria, ribosomes, nuclear atypia and nucleolus, and the immunohistochemistry result to analyse the relationship between the ultrastructural characteristics, expression of MMP-9 and VEGF and invasiveness in pituitary adenomas. Results The rough endoplasmic reticulum is increased, the ribosome is rich, the nuclei is irregular in shape and the nucleolu is huge in the cytoplasm of invasive pituitary adenomas, and the nucleolus occurrence rate, nuclear atypia rate, the nucleoplasm ratio and the number of mitochondrias in invasive pituitary adenomas were higher than those in the non-invasie group （ P 〈 O. 01 ）. MMP-9 and VEGF show strong positive expression in invasive pituitary adenomas. Conclusions Uhrastructural characteristics are related with the invasiveness of pituitary adenomas.