不同培养模式对微藻Chlorella vugaris代谢与蛋白质组分的影响

Effect of Culture Models on Metabolism and Protein Components of Microalgae Chlorella vulgaris

研究不同培养模式对小球藻蛋白合成的影响。分别采用自养、异养和混养模式培养小球藻生产蛋白质。结果为混养时藻细胞更易积累生物量,其比生长速率和产率高达0.526 d-1和0.318mg/(L·d),且其蛋白质质量分数和产率分别为35.6%和0.097 g/(L·d),自养时蛋白质质量分数却最高为52.1%;3种方式培养小球藻均含18种氨基酸,且必须氨基酸比例均超过总的40.0%;异养和混养方式下各氨基酸组成比例基本相似,而与自养时相比,Cys-s、Tyr和Met比例差异显著,Cys-s比例分别高了约78.57%,而Tyr及Met分别低27.59%和33.33%左右。营养方式显著影响小球藻蛋白质合成及氨基酸组成比例。

Biosynthesis of protein and amino acids by Chlorella vulgaris inoculated in different culture modes were studied,respectively.Chlorella strain was cultivated to synthesize protein in autotrophism,heterotrophism and mixotrophism models,respectively.The results were as follows：the mixotrophic algal cell could accumulate higher biomass,with a specific growth rate and productivity of 0.526 d-1 and 0.318 mg/（L·d）,respectively.Its high protein productivity was of 0.097 g/（L·d）,but the protein content in autotrophic Chlorella was the highest （52.1%）,which the algal protein contained 18 kinds of amino acids in the three models and the percentage of essential amino acids was 40.0% of the total;under heterotrophism and mixotrphism models,proportions of amino acids from the algal protein were rather similar,compared with the algae in autotrophism model,proportions of Cys-s,Tyr and Met were the significant difference,additionally Cys-s proportions in the previous two were about 78.57% higher than the later,while proportions of Tyr and Met were about 27.59% and 33.33% lower than the previous two,respectively.Consequently,biosynthesis of protein and proportion of the algal amino acids were both significantly influenced in the culture models.