鱿鱼墨黑色素铁对缺铁性贫血大鼠的影响

Effects of Squid Ink Melanin-Fe on Iron Deficiency Anemia in Rats

为了研究鱿鱼墨黑色素铁对缺铁性贫血大鼠的影响。采用56只断乳SD大鼠,随即选取8只饲喂正常饲料,另外48只饲喂低铁饲料28 d,造成缺铁性贫血模型后随即分为6组。其中3组分别灌胃不同剂量的鱿鱼墨黑色素铁(分别为6,12,18 mg/kg)21 d,阳性组分别灌胃硫酸亚铁和氯化铁(6 mg/kg),缺铁模型组和对照组分别每天灌胃等体积的去离子水共21 d。测定各组大鼠血液指标、脏器指数、血清铁、肝脏和脾脏的铁含量、抗氧化性能指标、血红细胞生成素、转铁蛋白受体和铁蛋白含量。结果表明,鱿鱼墨黑色素铁各剂量组和阳性组均可提高大鼠血红蛋白含量,提高血清、肝脏和脾脏铁含量、提高血清铁蛋白和促红细胞生成素含量,提高血清和肝脏抗氧化能力,降低血清总铁结合力和转铁蛋白受体含量,改善缺铁性贫血,比FeSO4和FeCl3的效果更佳。因此,鱿鱼墨黑色素铁可作为潜在的有效的补铁剂。

This study analyzed the effects of squid ink melanin-Fe （SM-Fe） on IDA in rats.Fiftysix weanling Sprague-Dawley male rats were used,and 48 rats were fed an iron-deficient diet for 4 weeks.Then,SM-Fe （dosage of iron 6 mg/kg BW,12 mg/kg BW and 18 mg/kg BW） were given to the IDA rats once a day for 3 weeks by intragastric administration,with FeCl3 and FeSO4 （dosage of iron 6 mg/kg BW） as positive controls.The IDA model group and the control groups were given distilled deionized water per day for 3 weeks.The content of hemoglobin （Hb）,serum iron （SI）,total iron binding capacity （TIB C）,serum ferritin （SF）,transferrin receptor （sTfR）,erythropoietin （EPO）,and iron contents in liver and spleen were measured.The results showed that the content of Hb,SI,SF,EPO,and iron contents in liver and spleen were significantly increased in the iron supplement groups （SM-Fe,FeCl3 and FeSO4） compared with the model group （P〈0.05）,while TIBC and sTfR were significantly decreased in the iron supplement groups compared with the model group （P〈 0.05）.In comparison with the FeCl3 and FeSO4 groups,higher bioavailability of iron and fewer side effects were observed in the SM-Fe group.The present study indicated that SM-Fe is an effective source of iron supplementation for IDA rats and might be exploited as a new iron fortifier.