不同柴胡剂量小柴胡汤对LPS诱导发热大鼠模型体温及血清IL-1β、IL-6、TNF-α的影响

Effect of Xiao-Chai-Hu Decoction with Different Doses of Bupleurum on Body Temperature and Content of IL-1β, IL-6, TNF-α in Serum Induced by LPS among Fever Rat Models

目的:观察不同柴胡剂量的小柴胡汤对LPS诱导发热大鼠模型体温及血清IL-1β、IL-6、TNF-α的影响,并探讨其作用机制。方法:50只SD大鼠随机分为5组:正常对照组、发热模型组、小柴胡汤(大)组、小柴胡汤(中)组、小柴胡汤(小)组。以20μg·kg-1剂量腹腔注射LPS制作发热模型;造模同时,给予相应的小柴胡汤水煎液或生理盐水。造模后每隔30 min测定体温(Ti),连续测量240 min,计算体温变化值ΔT(ΔT=Ti-T0)。最后1次测量体温后,取血,酶联免疫吸附法(ELISA)测定血清IL-1β、IL-6、TNF-α水平。结果:小柴胡汤(大)组在3 h、3.5 h、4 h此3个时间节点有明显降低大鼠体温的效果(P<0.01或P<0.05),这种降温效果在3.5 h、4 h两个时间节点优于小柴胡汤(中)组、小柴胡汤(小)组二组(P<0.01或P<0.05)。与发热模型组比较,3组小柴胡汤均可明显降低血清中IL-1β的含量(P<0.05),其中小柴胡汤(大)组效果显著(P<0.01)。与发热模型比较,小柴胡汤(大)组可明显降低血清中IL-6、TNF-α的含量(P<0.05)。结论:小柴胡汤具有降低LPS诱导发热大鼠模型体温与降低血清中的IL-1β、IL-6、TNF-α含量的功效,在一定范围内与方中柴胡剂量之间呈正相关剂量依赖关系。

This study was aimed to observe the effect of Xiao-Chai-Hu Decoction （XCHD） with different doses of Bupleurum on body temperature and content of IL-1β, IL-6 and TNF-α in serum among fever rat models induced by lipopolysaccharide （LPS） in order to and investigate its action mechanism. A total of 50 rats were randomly divid-ed into 5 groups, including the normal control group, fever model group, XCHD group （high-dose）, XCHD group （middle-dose）, and XCHD group （low-dose）. The fever rat model was established by intraperitoneal injection of LPS （20 μg/kg）. At the same time, all rats were given corresponding XCHD or saline solution by gavage. The body tem-perature of rats （Ti） was measured every 30 min for 240 min after the injection of LPS. Change values of body tem-perature △T（△T=Ti-T0） were calculated. After the last measurement of body temperature, blood was taken for the de-tection of IL-1β, IL-6 and TNF-α level in serum with ELISA. The results showed that the body temperature of XCHD group （high-dose） was obviously decreased at the time point of 3 h, 3.5 h, 4 h （P〉.01, or P〉.05）. This temperature reducing effect at the time point of 3.5 h and 4 h was better than the XCHD group （middle-dose） and XCHD group （low-dose, P〉.01, or P〉.05）. Compared with the fever model group, all three XCHD groups can＆amp;nbsp;obviously reduce the IL-1β level （P〉.05）. Among them, the XCHD group （high-dose） had obvious effect （P〈0.01）. Compared with the fever model group, the XCHD group （high-dose） can obviously reduce contents of IL-6 and TNF-α in serum （P〉.05）. It was concluded that XCHD can reduce fever induced by LPS in rats and decrease contents of IL-1β, IL-6 and TNF-α in serum. This effect has a positive dose-effect relationship with the dose of Bu-pleurum in a certain range.