藏药打箭菊的质量标准研究

Quality Standard of Pyrethri Tatsienenis Flos

目的：建立藏药打箭菊的质量标准。方法：采用显微、薄层色谱法建立其鉴别方法；参考2010年版《中国药典》（一部）相关方法测定其水分、总灰分、酸不溶性灰分和浸出物的含量；采用高效液相色谱法测定木犀草素的含量。结果：藏药打箭菊药材的显微、薄层色谱鉴别特征明显，专属性强；打箭菊药材中木犀草素含量为0.036%-0.104%（均值0.078%），水分为9.32%-15.82%（均值13.11%），总灰分为6.65%-8.29%（均值7.45%），酸不溶性灰分为0.23%-0.59%（均值0.42%），浸出物为21.42%-30.15%（均值24.86%）。结论：本文建立的方法操作简单、准确可靠、重复性良好，可用于控制打箭菊药材的质量。

This study was aimed to establish the quality standard of Pyrethri Tatsienenis Flos. The medical material was identified by the microscopy and the thin layer chromatography （ TLC ） methods . The moisture , total ash , acid-insoluble ash and alcohol-soluble extract were determined according to procedures recorded in the Chi-nese Pharmacopoeia （2010 edition）. The content of luteolin was determined by the HPLC method. The results showed a strong characteristic microscopic of Pyrethri Tatsienenis Flos , and its TLC identification had a good resolution with clear spots . The mass fractions of luteolin was 0 . 036%-0 . 104% （ average of 0 . 078%） , moisture was 9 . 32%-15 . 82% （ average of 13 . 11%） , total ash was 6 . 65%-8 . 29% （ average of 7 . 45%） , acid-insoluble ash was 0 . 23%-0 . 59% （ average of 0 . 42%） , and the extraction was 21 . 42%-30 . 15% （ average of 24 . 86%） . It was concluded that this established standard was simple to operate with good stability and reproducibility , which can be used for quality evaluation of Pyrethri Tatsienenis Flos .