HPLC法同时测定藏药川西千里光中的绿原酸、金丝桃苷和木犀草苷的含量

Simultaneous Determination of Chlorogenic Acid, Hyperoside and Luteoloside in Tibetan Medicine Senecio Solidagineus by HPLC

目的：本文建立了HPLC同时测定藏药川西千里光中绿原酸、金丝桃苷和木犀草苷3种成分的方法。方法：采用SinoChrom ODS-BP色谱柱（250 mm＆#215;4.6 mm，5μm），以乙腈（A）-0.1%磷酸溶液（B）为流动相，梯度洗脱，流速1 mL·min-1，检测波长355 nm，进样量10μL，柱温30益。结果：绿原酸、金丝桃苷、木犀草苷在30 min内均达到基线分离，各成分在其线性范围内均呈现良好的线性关系，线性范围分别为：0.6972-5.2290μg（r =0.9994）、0.0858-0.6438μg（r =1.0000）、0.0827-0.6204μg（r=1.0000）。平均加样回收率分别为：102.09%（RSD=0.99%）、101.17%（RSD=0.69%）、100.91%（RSD=1.01%）。结论：本方法操作简便、准确，重复性和稳定性好，可用于川西千里光的质量控制。

This study was aimed to establish a high-performance liquid chromatography （HPLC） method for simulta-neous determination of chlorogenic acid, hyperoside and luteoloside in Senecio solidagineus. Samples were analyzed on SinoChrom ODS-BP （250 mm ＆#237; 4.6 mm, 5 μm） with acetonitrile （A） and water containing 0.1% phosphate （B） as mobile phases for gradient elution at the flow rate of 1.0 mL＆#183;min-1. The detection wavelength was set at 355 nm. The injection volume was 10 μL. The column temperature was 30oC. The results showed that chlorogenic acid, hypero-side and luteoloside achieved baseline separation within 30 min. A good linearity was observed in the range of 0.697 2-5.229 0 μg （r = 0.999 4）, 0.0858-0.6438 μg （r = 1.000 0）, 0.082 7-0.620 4 μg （r = 1.000 0） for chloro-genic acid, hyperoside and luteoloside, respectively, with the average recoveries of 102.09% （RSD = 0.99%）, 101.17% （RSD = 0.69%）, and 100.91% （RSD = 1.01%）. It was concluded that the method was simple, accurate and reproducible, which can be used for the quality control of S. solidagineus.