摘要
目的建立假龙胆总多酚含量的测定方法。方法采用可见分光光度法测定假龙胆中总多酚的含量,并建立清除羟基自由基和超氧阴离子自由基两种抗氧化模型对假龙胆抗氧化能力进行评价。结果以没食子酸作为测定总多酚含量的对照品,在浓度0.05~0.3 mg(r=0.9999)范围内呈良好的线性关系,结果表明,总多酚含量平均值为7.63 mg,假龙胆提取液具有良好的抗氧化能力。结论此方法操作简便,重现性良好、稳定,可作为假龙胆总多酚的检测方法,并可作为天然抗氧化剂资源进行开发研究。
Objective To establish a method for the determination of total Polyphenol in Gentianella turkestanorum( Gand.) Holub. Methods The total Polypheno land in Gentianaceae was determined by visible spectrophotometry,and establish two kinds of anti-oxidation model-scavenging hydroxyl radicals and scavenging superoxide anion radicals-to evaluate Gentianella turkestanorum( Gand.) Holub. in its antioxidant ability. Results The method had a good linearity in the range of 0. 05 ~ 0. 3 mg( r = 0. 9999) with gallic acid as the reference substance. The results showed that an average total polyphenol content of 7. 63 mg,Gentianella turkestanorum( Gand.) Holub. extracts had good oxidation resistance. Conclusions This method was simple,with good reproducibility,and stability,and could be adopted as the standard method for detecting Gentianella turkestanorum( Gand.) Holub. total polyphenols. Gentianella turkestanorum( Gand.) Holub,as a natural anti-oxidant resources,is worth further research and development.
出处
《环球中医药》
CAS
2014年第2期103-106,共4页
Global Traditional Chinese Medicine
基金
新疆维吾尔药与特色天然药物创制及产业化示范项目(201130105-1)
重大公共卫生专项--全国第四次中药资源普查新疆(试点)项目(2100409)
关键词
假龙胆
总多酚
抗氧化
Gentianella turkestanorum(Gand.) Holub.
Total Polyphenol
Antioxidant activity