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17β-雌二醇对诱导绵羊输卵管上皮细胞β-防御素-1(SBD-1)基因表达的影响 被引量：1

Effects of 17β-estradiol on the Induced Sheep β-defensin-1 (SBD-1) Expression in the Ovine Oviduct Epithelial Cells
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摘要 研究了17β-雌二醇(E2)对培养的绵羊输卵管上皮细胞内SBD-1基因表达的影响.将不同剂量的17β-雌二醇加入传2代的绵羊输卵管上皮细胞内,分为实验组(10-6 mol/L组、10-7 mol/L组、10-8 mol/L组、10-9 mol/L组和10-10 mol/L组)和相应的对照组,分别处理2,6,12,24,48h,然后通过实时荧光定量RT-PCR技术,检测17β-雌二醇对绵羊输卵管上皮细胞内SBD-1mRNA的表达变化.结果显示:17β-雌二醇对绵羊输卵管上皮细胞中SBD-1mRNA的表达存在着时间效应和剂量上的依赖关系;17β-雌二醇可以上调绵羊输卵管上皮细胞中SBD-1mRNA表达,上调表达的最佳时间为6h,最佳浓度为10-8 mol/L(P<0.01). To investigate the effect of 17β-estradiol （E2） on β-defensin-1 gene expression in the ovine oviduct epithelial cells,the 2th generation of the sheep oviduct epithelial cells were treated in different time （2,6,12,24,48 h） with various dose of 17β-estradiol （10.6 ,10.7 ,10-8 ,10-9 ,10 -10 mol/L）,while a control group（without the 17β - estradiol,Control）, then the level of SBD-1 gene expression in the ovine oviduct epithelial cells was detected by real-time RT-PCR. The results showed that 17β-estradiol regulated SBD-1 mRNA expression in a time and dose-dependent manner. It is said that 17β-estradiol significantly up-regu- lated SBD-1 mRNA expression, and the maximal stimulatory effect of 17β-estradiol on the expression of SBD-1 mRNA was observed at the concentration of 10-8 mol/L after treatment for 6 h（P〈0.01）.
作者 包图雅 白萨日娜 曹贵方 凃勇 杜晨光
机构地区 内蒙古医科大学基础医学院 内蒙古农业大学动物组织胚胎与发育生物学研究室
出处 《内蒙古师范大学学报（自然科学汉文版）》 CAS 北大核心 2014年第1期81-85,共5页 Journal of Inner Mongolia Normal University(Natural Science Edition)
基金 国家自然科学基金资助项目(31060328)
关键词 17Β-雌二醇 输卵管上皮细胞 β-防御素-1(SBD-1) 上调 绵羊 基因表达 SBD-1 17β-estrodiol epithelial cells of fallopian tubes SBD-1 up-regulation sheep gene expression
分类号 Q492.5 [生物学—生理学]
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参考文献16

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共引文献55

同被引文献9

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内蒙古师范大学学报（自然科学汉文版）
2014年 第1期

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