内皮素1对家兔动脉粥样硬化斑块C反应蛋白表达的影响

Effect of Endothelin-1 on the Expression of C-reactive Protein in Rabbits with Atherosclerotic Plaque

[目的]观察由内皮素1激活的内皮素A受体对家兔动脉粥样硬化斑块C反应蛋白表达的影响。[方法]32只雄性新西兰大白兔完全随机设计分成基础组、模型组、BQ-123组以及辛伐他汀组，每组8只。饲喂10周后，采耳动脉血检测血清总胆固醇（TC）、三酰甘油（TG）、高密度脂蛋白胆固醇（HDL-C）以及低密度脂蛋白胆固醇（LDL-C）水平；采用免疫组织化学SP法检测主动脉标本中组织内皮素1（ET-1）、巨噬细胞及C反应蛋白（CRP）表达。[结果]模型组、BQ-123组及辛伐他汀组血清 TC、TG、LDL-C均高于基础组（ P均＜0．01）。BQ-123组及辛伐他汀组血清TC、TG、LDL-C均低于模型组（ P均＜0．01）。模型组、BQ-123组及辛伐他汀组血清HDL-C低于基础组（ P均＜0．01）。BQ-123组及辛伐他汀组血清HDL-C高于模型组（ P均＜0．01）。BQ-123组及辛伐他汀组家兔动脉粥样硬化斑块ET-1、巨噬细胞源泡沫细胞、CRP阳性表达均较模型组明显降低，BQ-123组家兔动脉粥样硬化斑块ET-1、巨噬细胞源泡沫细胞、CRP阳性表达又较辛伐他汀组进一步降低。[结论]ET-1激活的ET受体A（ETRA）可以导致家兔动脉粥样硬化斑块CRP表达的上调；选择性ETRA拮抗剂（ETRAA）可以预防家兔动脉粥样硬化进程的发展。

[Objective]To observe the effect of endothelin receptor A activated by endothelin-1（ET-1） on the expression of C-reactive protein（CRP） in rabbits with atherosclerotic plaque .[Methods] Thirty-two male New Zealand white rabbits were randomly divided into baseline group ,model group ,BQ-123 group and simv-astatin group with 8 rabbits in each group .After 10 weeks ,the peripheral blood was collected from ear artery to determine the levels of total cholesterol （TC） ,triglyceride（TG） ,high density lipoprotein cholesterol（HDL-C） and low density lipoprotein cholesterol（LDL-C） in serum .SP immunohistochemical staining was used to detect the expression of ET-1 ,macrophage and CRP in aorta .[Results] Serum levels of TC ,TG and LDL-C in model group ,BQ-123 group and simvastatin group were significantly higher than those in baseline group（all P〈0 .01） ,while serum levels of TC ,TG and LDL-C in BQ-123 group and simvastatin group were significant-ly lower than those in model group（all P〈0 .01） .Serum levels of HDL-C in model group ,BQ-123 group and simvastatin group were significantly lower than those in baseline group （all P〈0 .01） .Serum levels of HDL-C in BQ-123 group and simvastatin group were higher than those in model group （all P 〈0 .01） .Compared with model group ,the positive expression of ET-1 ,macrophage-derived foam cells and CRP in BQ-123 group and simvastatin group decreased obviously .Compared with simvastatin group ,the positive expression of ET-1 , macrophage-derived foam cells and CRP in BQ-123 group further decreased .[Conclusion] Endothelin receptor A activated by ET-1 can up regulate the expression of CRP protein in atherosclerotic plaque of rabbits .Selec-tive endothelin receptor A antagonist can prevent the development of atherosclerosis in rabbits .