地塞米松对ANIT诱导的胆汁淤积大鼠的治疗作用

Dexamethasone for the Treatment of Rats with ANIT-induced Cholestasis

[目的]探讨地塞米松对α-萘异硫氰酸（ANIT ）诱导的胆汁淤积大鼠的治疗作用。[方法]将健康雄性Wistar大鼠32只随机分为A、B、C、D四组。A组，仅灌花生油。B组，仅灌ANIT液。C组，灌ANIT液的同时予地塞米松（DEM ）处理。D组，灌ANIT液后24 h予DEM处理，每组8只。48 h后收集胆汁，计1 h胆汁量（BC）。取门静脉血检测总胆红素（TBIL），直接胆红素（DBIL）碱性磷酸酶（ALP），丙氨酸转氨酶（ALT ）的浓度。肝脏标本活检。[结果]B组各项指标明显高于A组（ P ＜0．01）。C、D两组 TBIL、DBIL指标低于B组（P ＜0．01），D组与C组两组无明显区别（P ＞0．05），D组略高于C组。B组ALP高于C组（P＜0．05），低于D组（ P＜0．01）。D组高于C组（ P ＜0．01）。B组ALT低于C组（ P ＜0．01）。C、D两组没有明显差别（P＞0．05）。B、D两组没有明显差别（P ＞0．05）。B、C、D三组中，BC值C组多于B、D组（P＜0．01）。D组多于B组（ P ＜0．01）。[结论]地塞米松有减黄作用，提示临床对药物性肝损引起的急性黄疸病例可早期予激素制剂干预。鉴于激素干预可能增加肝细胞损伤，大剂量应用应该谨慎。

[Objective]To explore the role of dexamethasone（DEM ） in the treatment of rats with cholesta-sis induced by α-isothiocyanate（ANIT） .[Methods]A total of 32 healthy adult male Wistar rats were randomly divided into group A ,B ,C and D with 8 rats in each group .Group A was treated with peanut oil only .Group B was infused with ANIT only .Group C was infused with ANIT and DEM concurrently .Group D was infused with DEM after infusion with ANIT for 24h .The 1h bile count（BC） was calculated .Portal venous blood was drawn to detect the levels of total bilirubin （TBIL） ,direct bilirubin（DBIL） ,alkaline phosphatase（ALP） and alanine transaminase（ALT） .Biopsy of liver samples was performed .[Results]The indicators of group B were obviously higher than those of group A（ P= 〈0 .01） .The TBIL and DBIL of group C and group D were low-er than those of group B（ P〈0 .01） ,but there was no significant difference between group D and group C （ P〉0 .05） ,and those in group B were slightly higher than those in group C .The ALP of group B was higher than that of group C（ P〈0 .05） ,but lower than that of group D（ P〈0 .01） ,and that of group D was higher than that of group C（ P〈0 .01） .The ALT of group B was lower than that of group C（ P〈0 .01） ,but there was no significant difference between group C and group D （ P〉0 .05） ,and there was no significant difference between group B and group D（ P〉0 .05） .The BC value of group C was higher than that of group B and D （ P〈0 .01） ,and that of group D was higher than that of group B （ P 〈0 .01） .[Conclusion] DEM can decrease jaundice .It suggests that early intervention with glucocorticoid agents should be taken for clinical cases of a-cute jaundice induced by drug-induced liver injury .Hormonal intervention may increase liver cell damage ,so high dose usage should be cautious .