神经-肿瘤相互作用对腺样囊性癌细胞侵袭、生存能力及基因表达的影响

Impact of neural microenvironment on invasion, survival and gene expression of adenoid cystic carcinoma cells

目的探讨神经微环境对腺样囊性癌(ACC)细胞侵袭、生存能力及基因表达的影响。方法通过免疫组织化学、ACC嗜神经侵袭体外模型、结合基因芯片技术,对临床病理标本及肿瘤细胞进行研究。结果32例ACC中有25例(78.1%)发生了嗜神经侵袭,其中实性型与筛孔型的嗜神经发生率较管状型高(P=0.03);神经组织与肿瘤细胞相互作用可以提高肿瘤细胞的生存能力、减少凋亡;通过基因芯片技术,在ACC-M与神经相互作用过程中筛选出369个表达上调的基因和920个表达下调的基因,其中许多基因已经证实参与调节细胞生长、凋亡、运动以及黏附等重要的生物过程。结论本研究初步揭示了神经组织与ACC细胞相互作用为肿瘤细胞提供了生存优势条件,为进一步对ACC嗜神经侵袭机制的研究提供了部分参考。

Objective To analyse the impact of neural microenvironment on invasion, survival and gene expression of adenoid cystic carcinoma（ACC） cells. Methods The immunohistochemical stain, ACC perineural invasion in vitro model, and Gene Chips were used to investigate the changing of invasion, survival ability and gene expression of ACC cells during the nerve-ACC cells interaction. Results PNI was identified in 25 of 32 ACC cases （78.1%）. The incidence of PNI was higher in solid and cribriform pattern of ACC. The nerve-ACC cells interaction could increase the survival ability and decrease the apoptotic activity of ACC cells. By GeneChips, we found 369 genes up-regulated and 920 genes down-regulated in the co-culture model. Conclusion The gene changing of ACC cell-nerve interaction might involve in the process of PNI.