胆汁对体外细粒棘球蚴原头节的作用观察

In vitro observation of the inhibitory effects of bile on Echinococcus granulosus protoscoleces

目的探讨不同浓度的胆汁对细粒棘球蚴原头节的生长作用及形态学影响。方法将体外培养的细粒棘球蚴原头节分别加入40%、60%、80%、100%的胆汁中体外孵育。0.1%伊红染色在倒置显微镜下检测原头节的活力及形态改变,实验重复三次;透射电子显微镜下(TEM)下观察胆汁作用后原头节表面超微结构改变。结果不同浓度的胆汁对原头节均有杀伤作用,其中浓度为100%和80%的胆汁对细粒棘球蚴原头节杀伤作用最明显。倒置显微镜下观察发现,不同浓度的胆汁作用细粒棘球蚴原头节后,原头节多呈外翻型,原头节顶突上的小钩排列紊乱,部分脱落,吸盘突起,变形,活动性减弱。随着浓度的增加,变化越明显。超微结构显示40%的胆汁作用3d后,原头节纤毛出现少量缺损,合胞体带排列较紊乱,合胞体带内出现散在少量的空泡和脂滴。60%胆汁作用3d后,合胞体带内微毛出现融合,腔内出现空泡和脂滴,数量多且大。结论胆汁作用后,可导致体外细粒棘球蚴原头节的形态发生不同程度的改变并有有抑制作用,然而关于胆汁在体内的疗效有待于进一步研究。

Objective To explore the in vitro effect of bile on Echinococcus granulosus protoscoleces and their morphol- ogy. Methods Protoscoleces of E. granulosus were incubated with bile at concentrations of 40%, 60%, 80%, and 100 %. Viability and changes in morphology were determined using 0.1 %eosin staining and light microscopy. The exper imeut was repeated three times. The uhrastruetural effects of protoseoleees were observed using transmission electron mi croscopy （TEM）. Results The protoscolicidal action of different concentrations of bile was observed. Clear protoscoli cldal action was observed with bile at concentrations of 100% and 80%. Morphological changes included contraction of the soma region and formation of blebs on the tegument. Treatment with 2 concentrations （60% and 40% ） of bile resul- ted in the formation of lipid droplet inclusions, the appearance of rounded mitochondria, and increased vacuolization of the germinal layer. The internal tissue was severely affected, resulting in the loss of its integrity and an increase in the num ber of lipid droplets. Conclusion Bile may lead to changes in the morphology of hydatid cysts and it has an inhibitory effect on E. granulosus. However, further investigation of the in vivo effectiveness of bile extracts is warranted.