益气消癥方对雌激素诱导子宫肌瘤模型豚鼠子宫组织血管生成的影响

Effect of Qi-Boosting Mass-Eliminating Formula on the Angiogenesis in Uterine Tissue of Guinea Pigs With Estrogen-Induced Uterine Fibroids

目的探讨益气消癥方治疗子宫肌瘤的可能作用机制。方法采用去势+雌激素诱导建立豚鼠子宫肌瘤模型,将68只造模成功豚鼠随机分为模型组(15只)、中药高剂量组(14只)、中药中剂量组(12只)、中药低剂量组(13只)及西药组(14只),并设正常对照组(15只)。去势术后第21日开始,中药高、中、低剂量组分别给予益气消癥方4g/kg、2g/kg、1g/kg干粉;西药组给予米非司酮片1.94mg/kg。各组灌胃容积为1ml/(100g·d),正常对照组及模型组给予等容积蒸馏水,连续12周。检测各组豚鼠子宫组织缺氧诱导因子-1α(HIF-1α)、血管内皮生长因子(VEGF)蛋白表达,并进行HIF-1α与VEGF相关性分析。结果模型组豚鼠子宫组织HIF-1α、VEGF蛋白表达显著高于正常对照组(P<0.05或P<0.01)。与模型组比较,中药高、中剂量组和西药组豚鼠子宫组织HIF-1α、VEGF蛋白表达明显降低(P<0.05或P<0.01);中药高剂量组豚鼠子宫组织HIF-1α蛋白表达显著低于中药中、低剂量组(P<0.01),中药高剂量组豚鼠子宫组织VEGF蛋白表达显著低于中药中、低剂量组和西药组(P<0.01)。HIF-1α与VEGF相关性分析显示,相关系数为0.930,P=0.000。结论益气消癥方可能是通过降低子宫组织HIF-1α、VEGF蛋白表达,抑制血管生成,从而达治疗子宫肌瘤的作用。

Objective To research the possible mechanism of Qi-Boosting Mass-Eliminating Formula for uterine fibroids. Methods The guinea pig models of uterine fibroids were established with castration and estrogen. Totally 68 guinea pig models were randomized into the model group （ n = 15 ）, Chinese medicine high-dose group （ n = 14）, Chinese medicine middle-dose group （ n = 12） , Chinese medicine low-dose group （ n = 13 ） and western medicine group （ n = 14）. The normal control group was established with 15 pigs in it. The Chinese medicine high-dose, mid- dle-dose and low-dose groups were given 4 mg/kg, 2 mg/kg and 1 mg/kg Qi-Boosting Mass-Eliminating Formula drypowder resp Tablets. Th model group were gavaged with the same volume of distilled water. The administration lasted tbr 12 weeks. The protein expressions of hypoxia-inducible factor-1α （HIF-lot） and vascular endothelial growth factor （VEGF） in uterine tissue in each group were observed. The correlation between HIF-1α and VEGF was analyzed. Results The protein expressions of HIF-1α and VEGF in the model group were significantly higher than those in the normal control group （ P 〈0. 05 or P 〈0.01 ）. Comparing with the model group, the protein expressions of HIF-1α and VEGF in the Chinese medicie high-dose and middle-dose groups and western medicine group were significantly decreased （P 〈 0.05 or P 〈 0. 0i ）. The protein expression of HIF-I c~ in the Chinese medicine high-dose group was significantly lower that in the Chinese medicine middle-dose and low-dose the Chinese medicine high-dose group was significantl dose groups and western medicine group （P 〈0. 01 ）. tion coefficient broids probably of 0 y lower groups （P 〈0.01 ）. than that in the Chin The protein expression of VEGF in ese medicine middle-dose and low- The correlation analysis of HIF-1α and VEGF showed a comelation 930 and P = 0. 000. Conclusion Qi-Boosting Mass-Eliminating Formula can treat uterine fi- reducing the protein expressions of HIF-1α and VEGF in uterine tissue and inhibiting angiogenesis.