摘要
试验旨在分析2013年新型重配A(H7N9)流感病毒分子流行病学特点。作者从GenBank数据库下载不同分离宿主的流感毒株NA全基因序列,应用分子生物学软件进行遗传进化分析。结果显示,2013年新型重配A(H7N9)流感毒株NA蛋白颈部氨基酸发生缺失,其唾液酸结合(HB)位点发生一定程度适应性变化,NA蛋白糖基化位点为7个,NA蛋白酶活性中心未发生变异。与A/Hangzhou/1/2013(H7N9)株中NA片段的核苷酸同源性较高的前10个序列均分离自亚洲5个国家的禽类,同源性达96%以上。至于此次新型重配A(H7N9)流感毒株NA基因是否是禽传给人尚不清楚,还有待进一步研究。
The assay was aimed to analyze the molecular epidemiological character of influenza A （H7N9） virus in 2013. The complete genome sequences of NA of influenza virus strains from different discrete host sources downloaded from the Gen- Bank were analyzed on genetic evolution by DNAStar software in this research. The results showed that, the neck amino acids of NA in influenza A （HTNg） virus in 2013 was deleted; HB site of NA took adaptive changes in certain degrees~ the active site of proteases of HA with seven glyeosylation sites was unchanged; the top ten high nucleotide homology strains with HA of A/ Hangzhou/1/2013（HTNg） had isolated from poultry in five Asian countries, the nucleotide homology was more than 96%. It was not clear whether the NA gene of novel reassortment influenza A （H7N9） virus strains was avian-to-human transmission and needed further study.
出处
《中国畜牧兽医》
CAS
北大核心
2014年第2期43-49,共7页
China Animal Husbandry & Veterinary Medicine
基金
新疆生产建设兵团博士资金(2011BB022、2012BB023)
国家级大学生创新项目(2013107570030)
