猪源植物乳杆菌的诱变选育及其对小鼠免疫性能的影响

Mutation Breeding of Porcine Lactobacillus plantarumand its Effects on Immune Function of Mice

为了获得1株具有较好耐酸耐胆盐、体外黏附率较高的植物乳杆菌,研究其对小鼠免疫性能的影响,试验将植物乳杆菌进行紫外诱变,对其耐酸和耐胆盐性能进行选育,将植物乳杆菌选育株BL-15冻干后,定量溶解,分3次口服(灌胃)BALB/c小鼠,采集血液、脾脏、粪便,处理后测定小鼠免疫性能指标的变化情况。结果表明,经过诱变后,从17株诱变株中成功筛选获得了1株耐酸耐胆盐较好、体外黏附率较高的植物乳杆菌BL-15,经小鼠口服后,提高了小鼠生长初期的脾脏指数及细胞因子IL-2的含量,加强免疫后肠内容物中SIgA含量与对照组相比,均差异显著(P<0.05)。说明该菌株可以诱导小鼠产生良好的黏膜免疫应答,提高小鼠的免疫性能。

To get a Lactobacillus plantarum strain with good acid resistance, cholate tolerance, and in vitro adhesion rate and study its effects on immune function of mice. After screening Lactobacillus plantarum by acid resistance and cholate toler ance after UV mutagenesis, we got BL-15 strain, which was cultured, freeze-dried, dissolved and administered to BALB/c mice orally （gastric perfusion） by three times. Blood, spleen and excrement were collected to detect changes in immune func- tion index. One Lactobacillus plantarum strain BL-15 was selected from 17 mutant strains, with higher acid resistance, eholate tolerance and in vitro adhesion rate. The experimental data of animal tests indicated that IL-2 level and spleen index of mice at early growth stage had been increased. Furthermore, the mice treated with BL-15 as the immunity errhancer revealed higher level of SIgA in intestinal contents compared with control （P〈0.05）. The results showed that Lactobacillus plantarum strain BL-15 had been proved to be able to elevate immune response and enhance immune function.