蛹虫草多糖对免疫抑制小鼠免疫功能的影响

Effect of Cordyceps militaris Polysaccharides on Immunity Function in Immunosuppressed Mice

90只雄性BALB/c小鼠随机分为6组,分别为健康对照组、模型对照组、阳性对照组和3个高、中、低蛹虫草多糖(CMP)剂量组。3个CMP组和模型对照组分别腹腔注射80mg/(kg·d)环磷酰胺,连续3d,然后3个CMP组分别灌胃给予17.5、35、70mg/kg CMP;健康对照组和模型对照组给予生理盐水;阳性对照组灌胃给予70mg/kg CMP,持续18d,于最后一次给药24h后,每组抽取3只小鼠用碳粒廓清试验检测巨噬细胞的吞噬指数;取3只小鼠用MTT法测定淋巴细胞增殖;其余小鼠称重后颈椎脱臼处死,采集内脏并称重,计算脾脏指数、胸腺指数。结果显示,3个CMP组以剂量依赖的方式增加小鼠的脾脏指数、胸腺指数、脾淋巴细胞增殖和巨噬细胞的吞噬指数,高剂量组的吞噬活性恢复到正常水平。结果表明,CMP可逆转环磷酰胺诱导的免疫器官萎缩,增强免疫抑制小鼠的细胞免疫和巨噬细胞功能。

90 male BALB/c mice were randomly divided into six groups. The mice in three experimental groups and model control group were given 80 mg/（kg . d） cyclophosphamide via intraperitoneal injection for 3 days. Three Cordyceps militaris polysaccharides （CMP） groups were respectively administrated 17.5, 35 and 70 mg/kg body weight CMP via gavage. The healthy control group and the model control group were given physiological saline, and the positive control group was orally ad- ministered 70 mg/kg CMP, lasting for 18 days. 24 h after the last administration, 3 mice of each group were detected macro phage phagocytic index by carbon clearance test; 3 mice were measured lymphocyte proliferation by MTT method;the remai ning mice were sacrificed by cervical dislocation after weighing, acquisition of visceral and weighing. The results showed that 3 groups of CMP in a dose-dependent manner increased spleen index, thymus index, spleen lymphocyte proliferation and macro- phage phagocytic index, and the phagocytic activity of high dose group returned to normal level. The results indicated that CMP could reverse the immune organ atrophy induced by cyclophosphamide in immunosuppressed mice, and enhance the cellu- lar immunity and macrophage function.