小花棘豆中毒对和田羊血清蛋白和血脂的影响

Effect of Oxytropis glabra DC Poisoning on Serum Protein and Blood Fat in Hetian Sheep

试验旨在探讨小花棘豆对和田羊血清蛋白和血脂的影响,进一步揭示小花棘豆对和田羊的毒性作用机理。将12只和田羊随机分为3组,即对照组、试验Ⅰ组和试验Ⅱ组,2个试验组分别按照10和20g/kg体重的剂量饲喂小花棘豆,至典型临床症状出现为止。试验前和试验期间每7d取血,检测和田羊血清蛋白与血脂指标,并进行血清蛋白电泳。经过42d的试验,各试验组和田羊的血清总蛋白(TP)、球蛋白(GLO)、α球蛋白和高密度脂蛋白(HDL-C)均无明显变化(P>0.05);β球蛋白、总胆固醇(TC)、低密度脂蛋白(LDL-C)和极低密度脂蛋白(VLDL-C)含量升高,白蛋白(ALB)、γ球蛋白和甘油三酯(TG)含量下降,部分组间差异显著或极显著(P<0.05或P<0.01);中毒羊血清白蛋白与球蛋白含量的比值(A/G)在整个试验期间均有降低的趋势。小花棘豆对试验羊的肝脏具有损害作用。

12 Hetian sheeps were randomly and equally divided into 3 groups （the control group, experimental group Ⅰ , group Ⅱ ） to investigate the effect of Oxytropis ggabra DC poisoning on serum protein and blood fat, then reveal the toxicity mechanism of Oxytropis glabra DC. These experimental groups of sheeps were fed daily contents of 10 or 20 g/（kg. BW） un til typical symptoms were observed. The contents of serum protein and blood fat were determined respectively before challenge test and at intervals of 7 days in this test. Meanwhile, the serum proteins were analysed by electrophoresis. After 42 days test, the results demonstrated that the contents of serum TP, GLO, a-globulin and HDL-C did not significantly change in each group （P〉0.05）; the contents of β-globulin, TC, LDL-C and VLDL-C in experimental groups were higher than normal control group, the contents of ALB, T-globulin and TG in experimental groups were lower than normal control group, with significant differences between the experimental groups （P〈0.05 or P 〈0.01） ; the content ratio of albumin to globulin（A/G） in test groups were decreased. The results showed that the sheeps＇ liver displayed different degrees of damage by poisoning of Oxyttopis glabra DC.