新疆小花棘豆对大鼠亚急性毒性及解毒剂解毒效果观察

Study on Subacute Toxicity and Detoxification Effect of Oxytropis glabra from Xinjiang in Mice

为了解新疆小花棘豆对大鼠肝脏组织结构的影响及解毒剂解毒效果观察,本试验将30只大鼠随机分为5组,即对照组饲喂基础日粮;试验1组饲喂添加小花棘豆的基础日粮;试验2组灌服从小花棘豆中提取的生物碱溶液,并饲喂基础日粮;试验3组饲喂添加小花棘豆的基础日粮,同时腹腔注射解毒剂;试验4组灌服从小花棘豆中提取的生物碱溶液,同时腹腔注射解毒剂,并饲喂基础日粮。单笼饲养,试验期21d。结果显示,试验1、2组大鼠肝脏、肾脏等均出现不同程度的充血、出血,肝小叶中大部分肝细胞肿胀,胞质出现空泡变性;脏器系数分析结果表明,解毒组与中毒组相比,差异显著(P<0.05)或极显著(P<0.01)。提示,解毒剂对小花棘豆及其生物碱引起的肝脏组织损伤具有一定的保护作用。

In order to understand the effect of Oxytropis glabra on the structure of liver tissue and observe the effect of an- tidote on the detoxification in mice, 30 mice were randomly divided into 5 groups, the control group was fed with basal diet, group 1 was fed with basal diet supplemented with Oxytropis glabra, group 2 was gavaged alkaloid solution extracted from Oxytropis glabra, group 3 was fed with basal diet supplemented with Oxytropis glabra and injected antidote into intraperitone- al simultaneously,group 4 was gavaged alkaloid solution extracted from Oxytropis glabra and injected antidote into intraperito- neal simultaneously. The mice were fed in single cage,and the trial lasted for 21 days. The results showed that the different de- gree of hyperemia and hemorrhage were appeared in liver and kidney tissue of groups 1 and 2. Most of the liver cells in hepatic lobule were swelled and vacuoles degenerated. The organ coefficient analysis showed that there were significant differences （P〈 0. 05） or extremely significant differences （P〈0. 01） between the detoxification groups and the poisoned groups. In couclusion, the antidote had a certain protective effect for liver tissue damage caused by Oxytropis glabra and its alkaloids.