三种来源的石榴多酚抗氧化活性比较

Comparative study on antioxidant activities of three sources of pomegranate polyphenols

目的:比较研究石榴皮、石榴汁、石榴渣多酚提取物的抗氧化活性。方法:通过测定多酚的总还原力、超氧阴离子自由基清除能力、羟自由基清除能力、DPPH·清除能力和对脂质过氧化的抑制能力五个指标评判和比较三种石榴多酚的体外抗氧化活性。结果:还原能力强弱为石榴渣多酚>石榴皮多酚>石榴汁多酚;对超氧阴离子自由基的清除能力顺序为:石榴渣多酚>石榴汁多酚>石榴皮多酚;清除羟自由基能力大小为:石榴汁多酚>石榴渣多酚>石榴皮多酚;对DPPH自由基的清除能力大小为:石榴汁多酚>石榴渣多酚>石榴皮多酚;对脂质过氧化的抑制能力强弱为:石榴渣多酚>石榴汁多酚>石榴皮多酚。结论:三种石榴多酚均具有较强的体外抗氧化和清除自由基活性,不同的评价体系,反应出的抗氧化能力高低不同。总体来说,石榴渣多酚和石榴汁多酚的抗氧化活性要高于石榴皮多酚,这可能与多酚组成有关。

Objective, To comparatively study the antioxidant activities of pomegranate polyphenols which were extracted from pomegranate peel, juice and residue respectively.Method, The antioxidant activities in vitro were measured by the methods of deoxidization capability, superoxide anion radical scavenging capacity, hydroxyl radical scavenging capacity, DPPH radical scavenging capacity and anti-lipid peroxidation capacity.Result, The order of deoxidization capabilities was,pomegranate residue polyphenols（PRPs） 〉 pomegranate peel polyphenols （PPPs） 〉 pomegranate juice polyphenols （PJPs）.The order of superoxide anion radical scavenging capabilities was：PRPs 〉 PJPs 〉 PPPs.The order of hydroxyl radical scavenging capabilities was.PJPs 〉 PRPs 〉 PPPs.The order of DPPH radical scavenging capabilities was. PJPs 〉 PRPs 〉 PPPs.The order of anti-lipid peroxidation capabilities was.PRPs 〉 PJPs 〉 PPPs.Conclusion.Three kinds of pomegranate polyphenols had significant antioxidant and free radical scavenging activities, and their antioxidant activity sequences were different according to the evaluation system.In general,the antioxidant activities of PRPs and PJPs were higher than PPPs,this may be related to the polyphenol compositions.