指纹图谱结合化学计量学分析用于多种大黄的鉴别及质量评价

Identification and Quality Evaluation of Radix et Rhizoma Rhei by HPLC Fingerprint and Chemometrics Analysis

目的建立大黄药材及饮片的高效液相色谱(HPLC)指纹图谱结合化学计量学分析法。方法样品经体积分数70%乙醇超声提取,0.45μm滤膜滤过,以0.1%磷酸-乙腈为流动相梯度洗脱,使用对照品和液质联用仪进行色谱峰识别,比较254及430 nm下各药材及饮片指纹图谱的异同,利用CHROMAP软件进行相似度、聚类和主成分分析。结果 254 nm下,3种正品大黄与非正品大黄均有各自的特征峰,430 nm下,特征峰减少,但共有峰的比例明显不同。254 nm下,3种正品大黄有24个共有峰,430 nm下有19个共有峰,指纹图谱经相似度、聚类或主成分分析,均可区分正品大黄与非正品大黄,并可对药材及饮片进行优劣评价。结论本法可用于区别不同种大黄药材及饮片并对其进行质量评价。

OBJECTIVE To develop the HPLC fingerprint of Radix et Rhizoma Rhei and chemometrics analysis method. METHODS The samples were extracted by ultrasonic with 70% ethanol, purified by 0.45 μm filter membrane, and eluted by 0. 1% phosphoric acid and acetonitrile in gradient mode; reference substances and LC-MS were used to identify the chromatogra- phy peaks ; the HPLC fingerprints of Radix et Rhizoma Rhei and its decoction pieces at 254 and 430 nm were compared and ana- lyzed with CHROMAP software. RESULTS Authentic and unauthentic Radix et Rhizoma Rhei all have their own characteristic peaks at 254 nm, but the characteristic peaks decreased at 430 nm and the proportion of common peaks areas were obviously dif- ferent. Twenty-five chromatographic peaks at 254 nm and 19 peaks at 430 nm were selected to distinguish the authentic and un- authentic Radix et Rhizoma Rhei by similarity analysis as well as to evaluate the quality of crude drugs and decoction pieces. CONCLUSION This method can be used for the identification and quality evaluation of different species of Radix et Rhizoma Rhei crude drugs and decoction pieces.