瘤果黑种草子总黄酮对慢性阻塞性肺病大鼠模型炎症的干预作用及机制研究

Effect of Total Flavonoids from Nigella glandulifera on Inflammation of Chronic Obstructive Pulmonary Disease Model Rats and Its Mechanism

目的观察瘤果黑种草子总黄酮对慢性阻塞性肺病大鼠模型炎症的作用及其机制。方法采用气管内滴入脂多糖(LPS)后雾化吸入木瓜蛋白酶制备慢性阻塞性肺病模型大鼠后,分别连续灌胃给予低、高剂量的瘤果黑种草子总黄酮、水煎液及氨茶碱20 d,模型组和正常组大鼠给予蒸馏水作为对照组。观察各组大鼠的一般状况,HE染色观察肺组织的病理形态学改变,取支气管肺泡灌洗液(BALF)进行炎性细胞分类,酶联免疫分析法测定支气管肺泡灌洗液上清液白细胞介素-8、肿瘤坏死因子-α和核因子-κB的含量水平。结果瘤果黑种草子总黄酮(71.5,286 mg·kg-1)可明显改善慢性阻塞性肺病模型大鼠的一般状况,对其肺组织的病理变化有不同程度的减轻;支气管肺泡灌洗液中的巨噬细胞、中性粒细胞所占比例显著下降(P<0.01),白细胞介素-8、肿瘤坏死因子-α和核因子-κB的含量水平均降低(P<0.01)。结论瘤果黑种草子总黄酮对慢性阻塞性肺病大鼠模型炎症具有抑制作用,其机制可能与减弱炎性细胞对脂多糖的反应,抑制炎症因子的合成和释放有关。

OBJECTIVE To investigate the effeet of total flavonoids from Nigella glamtulifera （TFNG） on inflammation of chronic obstructive puhnonary, disease （COPD）model rats and its mechanisms, METHODS The COPD model rats was established by intratra- cheal instillation of lipopolysaccha rides （LPS） with inhalation of papain. The model rats were administrated low and high dosage TFNG, water decoetion and aminophylline by ig for twenty consecutive （lays, the model and normal group with distilled water. The general situa- tion of rats were observed and the rats lung tissue were obtained and stained with HE for histopathological examination, inflammatory cells in bronchuoalveolus lavage fluid （BALF） were classified, the levels of inflammatory factors in BALF were measured by ELISA. RESULTS TFNG （71.5,286 mg · kg ^-1 ） could improve the general condition of COPD rats, and the pathologic changes of lung tissue were reduced in different degree; the macrophage and neutrophil cells percentage in BALF, and levels of IL-8 ,TNF-α and NF-κB in BALF were signitficantly decreased （P 〈0. 01 ）. CONCLUSION TFNG can inhibit the inflammation of COPD model rats. The potential mechanism may be related to reducing the reaction of inflammatory ceils to LPS, and inhibiting the synthesis and release of inflammatory cytokines.