高速逆流色谱分离纯化苦荞中芦丁、槲皮素

Isolation and Preparation of Flavones from the Bran of Tartary Buckwheat Using High Speed Counter Current Chromatography

利用乙醇溶液提取苦荞麸皮中总黄酮,将得到的苦荞黄酮粗提物用DA-201大孔树脂进行初步纯化。以得到的黄酮初步纯化产物为原料,应用高速逆流色谱对其中的芦丁和槲皮素进行分离、纯化。所用两相溶剂体系为乙酸乙酯-正丁醇(4∶1∶5,V/V),上相为固定相,下相为流动相,流速2 mL/min,转速850 r/min,检测波长254 nm。所得产物经高效液相色谱检测,结果表明得到芦丁纯度为99%,槲皮素纯度为94%。表明该法分离制备苦荞麸皮中芦丁、槲皮素简便,所得产物纯度高。

The bran of tartary buckwheat was extracted with 60% ethanol. A DA-201 macroporous resin column was applied in the purification of flavonoids from the extract. High speed counter current chromatography （HSCCC） method was used for preparative isolation and purification of rutin and quercetin from the preliminarily purified extracts. A two-phase solvent system composed of ethyl acetate, n-butyl alcohol and water （4： 1 ： 5, V/V） was used at a flow rate of 2.0 mL/min for HSCCC separation at a rotation speed of 850 r/min and the pooled eluates were detected at 254 nm. The purity of rutin and quercetin obtained was 99% and 94%, respectively, as detected by high performance liquid chromatography （HPLC）. These results indicate that this method is convenient and effective and can be used to extract and purify rutin and quercetin from tartary buckwheat bran.