响应面法优化海蓬子皂苷提取工艺条件与生物活性研究

Optimization of Extraction Conditions for Saponin from Salicornia herbacea by Response Surface Method and Its Biological Activity

响应面分析法优化海蓬子皂苷提取工艺,初步鉴定海蓬子皂苷的结构,并探讨其对α-葡萄糖苷酶与胰脂肪酶的体外抑制活性。海蓬子皂苷最佳提取条件为料液比1∶27(g/mL)、乙醇体积分数62%、超声时间30 min。在此条件下,海蓬子皂苷的提取量为10.14 mg/g;显色反应和红外光谱初步分析表明,海蓬子皂苷为四环三萜类皂苷;体外实验表明,经纯化的海蓬子皂苷提取物对α-葡萄糖苷酶和胰脂肪酶均有较强抑制作用,并呈剂量效应,对α-葡萄糖苷酶、胰脂肪酶的抑制率分别达到68.20%和84.28%。该结果提示海蓬子皂苷可能具有阿卡波糖样的降血糖机制以及降血脂的功效。

Response surface methodology was used to optimize the extraction process for saponins from young leaves of Salicornia herbacea. The saponions extracted were structurally characterized and evaluated for inhibitory effect on α-glucosidase and pancreatic lipase in vitro. Our results indicated that the optimal extraction conditions were material-to- liquid ratio of 1：27 （g/mL）, ethanol concentration （V/V） of 62%, and ultrasonification time of 30 min. Under these extraction conditions, maximum yield of saponins of 10.14 mg/g was obtained. Color reaction and infrared spectral analysis showed that the basic structure of saponins from Salicornia herbacea was tetracyclic triterpene saponin. The purified saponins had a strong inhibitory effect on both α-glycosidase and pancreatic lipase in vitro in a dose-dependent manner, and the maximum inhibitory rates were 68.20% and 84.28%, respectively. These results suggest that saponins from Salicornia herbacea may have similar hypoglycemic mechanism and hypolipidemic effect to acarbose.