摘要
白苞蒿Artemisia lactiflora是一种重要的药用植物。对已分离获得的54株白苞蒿内生真菌粗提物分别采用MTT法和DPPH法进行体外抗肿瘤和抗氧化活性筛选,并采用分子生物学方法对筛选出的活性内生真菌进行鉴定。结果显示,共计10株(18.5%)白苞蒿内生真菌粗提物对人骨髓白血病细胞系(HL-60)、人乳腺癌细胞系(MCF-7)和人前列腺癌细胞系(PC-3)生长的抑制活性较好,其中以链格孢属GYBH47菌株粗提物的抗肿瘤活性最佳,对3种肿瘤细胞株的生长均有抑制作用;共计2株(3.7%)白苞蒿内生真菌粗提物的抗氧化活性较好,其中,拟茎点霉属GYBH42菌株粗提物同时具有抗氧化活性和细胞毒活性。表明贵州白苞蒿内生真菌是寻找有价值的生物活性成分的潜在资源,其生物活性成分值得进一步研究和开发。
Artemisia lactiflora is an important medicinal plant in China. The antitumor and antioxidant activities of the extracts of 54 endophytic fungi from the plant were screened via MTT assay and DPPH scavenging radical assay, respectively. The bioactive strains were identified based on similarity of 5.8S gene and internal transcribed spacer (ITS) sequences. The results showed that ex- tracts from ten ( 18.5% ) isolates exhibited antitumor activity, and which from two (3.7%) isolates exhibited antioxidant activity. The Alternaria sp. GYBH47 strain was simultaneously having antagonistic activity against HL-60 leukemia, MCF-7 breast and COLO205 colon cell lines, and Phomopsis sp. GYBH42 strain having cytotoxic and antioxidant activities. The results indicated that endophytic fungi from Artemisia lactiflora are potential resources to find valuable bioactive components.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2014年第3期438-441,共4页
China Journal of Chinese Materia Medica
基金
贵阳市社会发展与民生科技计划项目(筑科合同[2012103]67号)
贵州省科技厅农业攻关项目(黔科合NY[2013]3042号
黔科合NY[2013]3044号)
贵州省科技厅国际合作项目(黔科合外G字[2012]7006号)
贵州省科技创新人才团队建设项目(黔科合人才团队[2012]4007号)
关键词
白苞蒿
内生真菌
细胞毒活性
抗氧化活性
分子鉴定
Artemisia lactiflora
endophytic fungi
cytotoxic activity
antioxidant activity
molecular identification