痰瘀同治方对痰瘀互结证冠心病小型猪心功能的改善作用

Effect of formula of removing both phlegm and blood stasis in improving cardiac function of Chinese mini-swine with coronary heart disease of phlegm-stasis cementation syndrome

目的:观察痰瘀同治方对痰瘀互结证冠心病小型猪心功能的影响。方法:将36只中国小型猪随机分为正常对照组、模型组、丹蒌片组和痰瘀同治方高、中、低剂量组(生药2.0,1.0,0.5 g·kg-1),每组6只;除对照组外,其他各组均给予高脂饲料喂养2周后,采用介入技术球囊损伤冠状动脉左前降支内皮,术后继续高脂饲料喂养8周,制备小型猪痰瘀互结证冠心病模型;术后给药8周,观察心肌缺血程度的变化,并采用超声心动图和无创血流动力学方法检测心脏功能和结构的改变。结果:与对照组比较,模型组心肌缺血程度明显增加,无创血流动力学参数中CO,SV和LCW均明显降低,而SVR则明显增加(P<0.05或P<0.01);超声心动图显示IVSd,LVPWs,EF和FS明显降低,LVIDs则明显增加(P<0.05或P<0.01);与模型组比较,痰瘀同治方能够减少心肌缺血程度,增强心功能;改善缺血导致的心肌结构和功能改变(P<0.05或P<0.01)。结论:痰瘀同治方对痰瘀互结证冠心病小型猪心功能具有明显的改善作用;采用临床心功能的检测方法能正确评价冠心病小型猪心肌缺血后心功能的改变,缩小临床与基础实验研究之间的差距。

Objective： To evaluate that the effect of formula of removing both phlegm and blood stasis in improving cardiac function of Chinese mini-swine with coronary heart disease of phlegm-stasis cementation syndrome. Method： Totally 36 Chinese miniswine were randomly divided to six groups： the normal control group, the model group, the Danlou tablet group, and Tanyu Tongzhi Fang （TYTZ） groups with doses of 2. 0, 1.0 and 0. 5 g . kg - 1, with six in each group. Except for the normal control group, all of other groups were fed with high-fat diet for 2 weeks. Interventional balloons are adopted to injure their left anterior descending artery endothelium. After the operation, they were fed with high-fat diet for 8 weeks to prepare the coronary heart disease model of phlegm-stasis cementation syndrome. After the operation, they were administered with drugs for 8 weeks. The changes in the myocardial ischemia were observed. The changes in the cardiac function and structure were detected by cardiac ultrasound and noninvasive hemodynamic method. Result： Compared with the normal control group, the model group showed significant increase in myocardial ischemia and SVR and obvious decrease in CO, SV and LCW in noninvasive hemodynamic parameters （P 〈 0.05 or P 〈 0. 01 ）. The ultrasonic cardiogram indicated notable decrease in IVSd, LVPWs, EF and FS, and remarkable increase in LVIDs （P 〈0. 05 or P 〈0. 01 ）. Compared with the model group, TYTZ could reduce the myocardial ischemia, strengthen cardiac function, and improve the abnormal cardiac structure and function induced by ischemia （P 〈 0. 05 or P 〈 0. 01 ）. Conclusion： TYTZ shows a significant effect in improving cardiac function of Chinese mini-swine with coronary heart disease of phlegm-stasis cementation syndrome. The clinical cardiac function detection method could be adopted to correctly evaluate the changes in the post-myocardial ischemia cardiac function, and narrow the gap between clinical application and basic experimental studies.