摘要
该研究利用人脐静脉内皮细胞模型研究丹参脂溶性成分对血管新生的调节作用,并探讨其可能的作用机制。通过建立的细胞模型,确定丹参脂溶性成分对内皮细胞增殖活性和迁移能力的影响;随后利用实时荧光定量PCR技术检测药物作用后血管新生相关细胞因子VEGF-A,VEGF-C和MMP-9的基因表达变化。结果表明,5 mg·L^(-1)的丹参脂溶性成分抑制内皮细胞的增殖和迁移,同时VEGF-A,VEGF-C和MMP-9基因的表达被抑制。表明丹参脂溶性成分可能通过抑制VEGF-A和MMP-9基因的表达来抑制内皮细胞的增殖和迁移,从而达到抑制血管新生的作用。
In this study, the human umbilical vein endothelial cell model was used to study the regulating effect of lipophilic components in Salvia miltiorrhiza on angiogenesis, and explore its possible mechanism. The cell model was established to determine the effect of lipophilic components in S. miltiorrhiza on the proliferative activity and migration capacity of endothelial cells. Then the realtime fluorescence quantification PCR technology was applied to detect the changes in the gene expressions of angiogenesis-related cyto- kines VEGF-A, VEGF-C and MMP-9. The results showed that 5 mg . L-1 lipophilic components in S. miltiorrhiza could inhibit the proliferation and migration of endothelial ceils, and reduce the expression of VEGF-A and MMP-9 genes. It indicated that lipophilic components in S. miltiorrhiza may inhibit the proliferation and migration of endothelial cells by inhibiting the expression of VEGF-A and MMP-9 genes, so as to show the inhibitory effect on angiogenesis.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2014年第4期744-747,共4页
China Journal of Chinese Materia Medica
基金
国家自然科学基金重点项目(81130066)
国家"重大新药创制"科技重大专项(2013ZX09507005)
关键词
丹参脂溶性成分
血管新生
人脐静脉内皮细胞
实时荧光定量PCR
血管内皮生长因子
lipophilic components in Salvia miltiorrhiza
angiogenesis
human umbilical vein endothelial cells
Real-time fluorescence quantification PCR
vascular endothelial growth factor