可见光谱结合主成分回归法同时测定葡萄干中的单糖类化合物

Simultaneous Determination of Simple Sugars in Raisins By Visible Spectroscopy with Multivariate Calibration Method

在硫酸介质,于温度100℃条件下,葡萄糖、果糖和木糖生成糠醛或糠醛衍生物,可与咔唑反应生成有色络合物。但络合物的吸收光谱重叠较严重,难以对单一组分进行测定。本文采用主成分回归法(PCR)解决光谱重叠的标准混合试样并建立校准模型。将此模型应用于葡萄糖、果糖和木糖模拟混合试样的同时测定。对模拟混合试样,回收率分别为99.2%,99.3%和100.7%。该方法应用于葡萄干样品中葡萄糖、果糖、木糖的测定,结果令人满意。

In sulfuric acid medium,sugars, such as glucose,fructose, and xylose, were reacted with the acid and furfural and derivatives were generated at 100 ℃. Subsequently, coloured products was produced when carbazole, as a chromogenic reagent, was added. It was found that the absorption spectra of the coloured complexes of glucose, fructose, xylose with the carbazole were seriously overlapped, and it was difficult to determine them individually. In this work principal component regression（PCR） was applied to resolve the overlapping spectra of a set of mixtures and the calibration model was built. Then this calibration model was mixtures with average further applied for the results. applied for predicting the contents of glucose, fructose and xylose in synthetic recoveries of 99.2% ,99.3% and 100.7% ,respectively. The proposed merhod was determination of glucose, fructose and xylose in raisin samples with satisfactory