克拉霉素与苯基荧光酮的荷移反应及其测定

Spectrophotometric Determination of Clarithromycin Based on the Charge Transfer Reaction between Clarithromycin and Phenylfluorone

本文建立了一种测定克拉霉素的荷移分光光度法，克拉霉素与苯基荧光酮（PF）在乙醇溶液中发生荷移反应，荷移络合物在波长519nm处有最大吸收，其表观摩尔吸光系数为1．70×10-4L·mol-1·cm-1荷移络合物的组成比为1：1，稳定常数为2．86×10-4，药物浓度在5．0×10-6～7．0×10mol／L范围内服从比耳定律，当克拉霉素浓度为4．0×10-5mol／L时，8次测定结果的相对标准偏差（RSD）为0．46％，检出限为6．0×10-7mol／L。利用该法测定了克拉霉素胶囊中有效成分的含量，回收率大于98％以上。

A chage-transfer based spectophotometric method for clarithromycin is established. Clarithromycin reacts with phenylfluorone in ethanol to produce a charge-transfer complex, which has a maximum absorption at 519 mm and an apparent molar absorption coefficient of 1.70 X 104 L- mol-1 cm-1. The complexation ratio is 1 ： 1 and the stability constant is 2.86 × 104. A linear relationship is found in the concentration range of 5.0 × 10-6-7. 0 × 10 5 mol· L-1 , and the detection limit is 6.0 × 10-7 mol- L-1 with RSD 0.46%. The recovery of clarithromycin capsule samples is 98%.